Application of a TLC chemical method to detection of cyclotides in plants
Xu WenYan1,2; Tang Jun1,2; Ji ChangJiu1,2; He WenJun1,2; Tan NingHua1
2008-06-01
发表期刊CHINESE SCIENCE BULLETIN
ISSN1001-6538
卷号53期号:11页码:1671-1674
摘要Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC coloring plots with G-250 and, ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.
关键词Cyclotides Tlc Chemical Detection Method Coomassie Brilliant Blue G-250
学科领域Multidisciplinary Sciences
DOI10.1007/s11434-008-0178-8
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000256188200009
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/1857
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Xu WenYan,Tang Jun,Ji ChangJiu,et al. Application of a TLC chemical method to detection of cyclotides in plants[J]. CHINESE SCIENCE BULLETIN,2008,53(11):1671-1674.
APA Xu WenYan,Tang Jun,Ji ChangJiu,He WenJun,&Tan NingHua.(2008).Application of a TLC chemical method to detection of cyclotides in plants.CHINESE SCIENCE BULLETIN,53(11),1671-1674.
MLA Xu WenYan,et al."Application of a TLC chemical method to detection of cyclotides in plants".CHINESE SCIENCE BULLETIN 53.11(2008):1671-1674.
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