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题名: Application of a TLC chemical method to detection of cyclotides in plants
作者: Xu WenYan1, 2; Tang Jun1, 2; Ji ChangJiu1, 2; He WenJun1, 2; Tan NingHua1
刊名: CHINESE SCIENCE BULLETIN
关键词: cyclotides ; TLC chemical detection method ; coomassie brilliant blue G-250
英文摘要: Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC coloring plots with G-250 and, ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.
出版日期: 2008-06-01
卷号: 53, 期号:11, 页码:1671-1674
DOI标识: 10.1007/s11434-008-0178-8
语种: 英语
ISSN号: 1001-6538
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/1857
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Xu, WY; Tang, J; Ji, CJ; He, WJ; Tan, NH.Application of a TLC chemical method to detection of cyclotides in plants,CHINESE SCIENCE BULLETIN,2008,53(11):1671-1674
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