Application of a TLC chemical method to detection of cyclotides in plants

doi:10.1007/s11434-008-0178-8

	Application of a TLC chemical method to detection of cyclotides in plants
	Xu WenYan 1,2; Tang Jun 1,2; Ji ChangJiu 1,2; He WenJun 1,2; Tan NingHua 1
	2008-06-01
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	53 期号:11 页码:1671-1674
摘要	Iodine, Dragendorff's reagent, ninhydrin and Coomassie brilliant blue G-250 reagents were used to detect cyclotides from proteins, cyclopeptides, linear-peptides and amino acids in the thin layer chromatography (TLC). Cyclotides could be distinguished from the others by comparing the TLC coloring plots with G-250 and, ninhydrin reagents. With this method, cyclotides were discovered in Viola labridorica, V. tricolor, V. hamiltoniana, Momordica charantia, and M. cochinchinensis and over 10 cyclotides were isolated, three of which were determined as known cyclotides cycloviolacin O2, kalata B1 and vary peptide A.
关键词	Cyclotides Tlc Chemical Detection Method Coomassie Brilliant Blue G-250
学科领域	Multidisciplinary Sciences
DOI	10.1007/s11434-008-0178-8
收录类别	SCI
语种	英语
WOS记录号	WOS:000256188200009
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/1857
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu WenYan,Tang Jun,Ji ChangJiu,et al. Application of a TLC chemical method to detection of cyclotides in plants[J]. CHINESE SCIENCE BULLETIN,2008,53(11):1671-1674.
APA	Xu WenYan,Tang Jun,Ji ChangJiu,He WenJun,&Tan NingHua.(2008).Application of a TLC chemical method to detection of cyclotides in plants.CHINESE SCIENCE BULLETIN,53(11),1671-1674.
MLA	Xu WenYan,et al."Application of a TLC chemical method to detection of cyclotides in plants".CHINESE SCIENCE BULLETIN 53.11(2008):1671-1674.

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