Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China

doi:10.4238/2014.October.8.3

	Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China
	Yang, Y. -W.1; Yang, Z. -Y.2; Yan, M. -R.1; Qian, Z. -G.1; Guan, K. -Y.2; Qian,ZG (reprint author),Yunnan Coll Tradit Chinese Med,Ctr Reproducing Fine Varieties Chinese Med Plants,Kunming,Yunnan,Peoples R China.
	2014
发表期刊	GENETICS AND MOLECULAR RESEARCH
ISSN	1676-5680
卷号	13 期号:4 页码:8220-8224
摘要	Twenty-four microsatellite markers were isolated from the genomic DNA of Amomum tsaoko Crevost et Lemaire, an important economic plant in China, using the method fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 60 individuals from three populations in Yunnan Province, China, and nine of them were polymorphic. The number of alleles per polymorphic locus was 2, and the expected and observed heterozygosities ranged from 0.224 to 0.513, and from 0.050 to 0.600, respectively. Among nine microsatellite markers with polymorphism, five showed significant deviation from Hardy-Weinberg equilibrium (P < 0.01), probably due to anthropic selection and short-cloning history in cultivation. No significant linkage disequilibrium was detected between loci in our analysis. These polymorphic microsatellite markers will facilitate further studies of gene flow, population structure, identification of cultivated variety, and evaluation of germplasm resources.
关键词	Amomum Tsaoko Fiasco Zingiberaceae Polymorphism Genetic Structure
学科领域	Biochemistry & Molecular Biology ; Genetics & Heredity
DOI	10.4238/2014.October.8.3
收录类别	SCI
语种	英语
WOS记录号	WOS:000345119800016
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/18509
专题	资源植物与生物技术所级重点实验室
通讯作者	Qian,ZG (reprint author),Yunnan Coll Tradit Chinese Med,Ctr Reproducing Fine Varieties Chinese Med Plants,Kunming,Yunnan,Peoples R China.
作者单位	1.Yunnan Coll Tradit Chinese Med, Ctr Reproducing Fine Varieties Chinese Med Plants, Kunming, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Yang, Y. -W.,Yang, Z. -Y.,Yan, M. -R.,et al. Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China[J]. GENETICS AND MOLECULAR RESEARCH,2014,13(4):8220-8224.
APA	Yang, Y. -W.,Yang, Z. -Y.,Yan, M. -R.,Qian, Z. -G.,Guan, K. -Y.,&Qian,ZG .(2014).Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China.GENETICS AND MOLECULAR RESEARCH,13(4),8220-8224.
MLA	Yang, Y. -W.,et al."Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China".GENETICS AND MOLECULAR RESEARCH 13.4(2014):8220-8224.

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