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题名: Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China
作者: Yang, Y. -W.1; Yang, Z. -Y.2; Yan, M. -R.1; Qian, Z. -G.1; Guan, K. -Y.2
刊名: GENETICS AND MOLECULAR RESEARCH
出版日期: 2014
卷号: 13, 期号:4, 页码:8220-8224
关键词: Amomum tsaoko ; FIASCO ; Zingiberaceae ; Polymorphism ; Genetic structure
学科分类: Biochemistry & Molecular Biology ; Genetics & Heredity
DOI: 10.4238/2014.October.8.3
通讯作者: Qian,ZG (reprint author),Yunnan Coll Tradit Chinese Med,Ctr Reproducing Fine Varieties Chinese Med Plants,Kunming,Yunnan,Peoples R China.
文章类型: Article
英文摘要: Twenty-four microsatellite markers were isolated from the genomic DNA of Amomum tsaoko Crevost et Lemaire, an important economic plant in China, using the method fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 60 individuals from three populations in Yunnan Province, China, and nine of them were polymorphic. The number of alleles per polymorphic locus was 2, and the expected and observed heterozygosities ranged from 0.224 to 0.513, and from 0.050 to 0.600, respectively. Among nine microsatellite markers with polymorphism, five showed significant deviation from Hardy-Weinberg equilibrium (P < 0.01), probably due to anthropic selection and short-cloning history in cultivation. No significant linkage disequilibrium was detected between loci in our analysis. These polymorphic microsatellite markers will facilitate further studies of gene flow, population structure, identification of cultivated variety, and evaluation of germplasm resources.
类目[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
收录类别: SCI
项目资助者: TRF/BIOTEC program Biodiversity Research and Training Grant [BRT R_251181, BRT R_253012] ; USDA-ARS ; Systematic Mycology and Microbiology Laboratory (SMML) ; Mushroom Research Foundation, Chiang Rai Province ; Mae Fah Luang University [56101020032] ; Distinguished Scientist Fellowship Program (DSFP), King Saud University, Saudi Arabia ; Chinese Academy of Sciences [2013T2S0030]
语种: 英语
WOS记录号: WOS:000345119800016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18509
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Yunnan Coll Tradit Chinese Med, Ctr Reproducing Fine Varieties Chinese Med Plants, Kunming, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China

Recommended Citation:
Yang, Y. -W.,Yang, Z. -Y.,Yan, M. -R.,et al. Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae), an economically important plant in China[J]. GENETICS AND MOLECULAR RESEARCH,2014,13(4):8220-8224.
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