Comparison of Natural and Synthetic alpha-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural alpha-Tocopherol
Wang, Xiaoxiao1; Cheng, Yongxian2; Chen, Xiaoshuang1; Liao, Jinhua1; Yang, Juan1; Hu, Xujia1; Hu,XJ (reprint author),Kunming Univ Sci & Technol,Fac Life Sci & Technol,Kunming 650500,Peoples R China.
2014-08-01
Source PublicationASIAN JOURNAL OF CHEMISTRY
ISSN0970-7077
Volume26Issue:16Pages:5279-5282
Contribution Rank第二
AbstractA validated HPLC with UV method was developed to evaluate the difference between natural (RRR) alpha-tocopherol and synthetic (all-rac) alpha-tocopherol in rat plasma by an intragastric administrated does of alpha-tocopherol acetate. The direct extraction of analytes in two steps with methanol/isopropanol (8:2, v/v) and n-hexane. This method employed a Kromasil C-18 column and methanol/water (98:2, v/v) as mobile phase, at a flow rate of 1 mL min(-1). The limits of detection (LOD) were 0.12 mu g mL(-1) for DL-alpha-tocopherol acetate and 0.1 mu g mL(-1) for DL-alpha-tocopherol, respectively. The recoveries of the method were near 95%. Intra-assay and inter-assay precision were, respectively 2.3-5.5 % and 2.1-7.9 %. The method was employed to evaluate natural (RRR) and synthetic (all rac) alpha-tocopherol in rat plasma. Meanwhile, the alpha-tocopherol content in Euryale ferox seed was measured. The results obtained from this study demonstrated that natural alpha-tocopherol was less accumulated than synthetic alpha-tocopherol and safe for long term use. After measurement, Euryale ferox seed is a potentially rich source of natural tocopherols and could be used as antioxidants in food for nutraceutical purpose.
KeywordAlpha-tocopherol Vitamin e Hplc Rat Plasma Euryale Ferox Seed
Subject AreaChemistry, Multidisciplinary
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000343767000074
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18485
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorHu,XJ (reprint author),Kunming Univ Sci & Technol,Fac Life Sci & Technol,Kunming 650500,Peoples R China.
Affiliation1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiaoxiao,Cheng, Yongxian,Chen, Xiaoshuang,et al. Comparison of Natural and Synthetic alpha-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural alpha-Tocopherol[J]. ASIAN JOURNAL OF CHEMISTRY,2014,26(16):5279-5282.
APA Wang, Xiaoxiao.,Cheng, Yongxian.,Chen, Xiaoshuang.,Liao, Jinhua.,Yang, Juan.,...&Hu,XJ .(2014).Comparison of Natural and Synthetic alpha-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural alpha-Tocopherol.ASIAN JOURNAL OF CHEMISTRY,26(16),5279-5282.
MLA Wang, Xiaoxiao,et al."Comparison of Natural and Synthetic alpha-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural alpha-Tocopherol".ASIAN JOURNAL OF CHEMISTRY 26.16(2014):5279-5282.
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