Hu,XJ (reprint author),Kunming Univ Sci & Technol,Fac Life Sci & Technol,Kunming 650500,Peoples R China.
A validated HPLC with UV method was developed to evaluate the difference between natural (RRR) alpha-tocopherol and synthetic (all-rac) alpha-tocopherol in rat plasma by an intragastric administrated does of alpha-tocopherol acetate. The direct extraction of analytes in two steps with methanol/isopropanol (8:2, v/v) and n-hexane. This method employed a Kromasil C-18 column and methanol/water (98:2, v/v) as mobile phase, at a flow rate of 1 mL min(-1). The limits of detection (LOD) were 0.12 mu g mL(-1) for DL-alpha-tocopherol acetate and 0.1 mu g mL(-1) for DL-alpha-tocopherol, respectively. The recoveries of the method were near 95%. Intra-assay and inter-assay precision were, respectively 2.3-5.5 % and 2.1-7.9 %. The method was employed to evaluate natural (RRR) and synthetic (all rac) alpha-tocopherol in rat plasma. Meanwhile, the alpha-tocopherol content in Euryale ferox seed was measured. The results obtained from this study demonstrated that natural alpha-tocopherol was less accumulated than synthetic alpha-tocopherol and safe for long term use. After measurement, Euryale ferox seed is a potentially rich source of natural tocopherols and could be used as antioxidants in food for nutraceutical purpose.
1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China
Wang, Xiaoxiao,Cheng, Yongxian,Chen, Xiaoshuang,et al. Comparison of Natural and Synthetic alpha-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural alpha-Tocopherol[J]. ASIAN JOURNAL OF CHEMISTRY,2014,26(16):5279-5282.