| Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry | |
Seeber, Elke1; Winterfeld, Grit2,3; Hensen, Isabell2,3,4; Sharbel, Timothy F.5; Durka, Walter6; Liu, Jianquan7; Yang, Yong-Ping8 ; Wesche, Karsten1,4; Seeber,E (reprint author),Senckenberg Museum Nat Hist Goerlitz,Dept Bot,POB 300 154,D-02806 Goerlitz,Germany.; elke.seeber@senckenberg.de
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| 2014-09-01 | |
| 发表期刊 | BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
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| ISSN | 0024-4074 |
| 卷号 | 176期号:1页码:22-35 |
| 摘要 | Polyploidy is a fundamental mechanism in evolution, but is hard to detect in taxa with agmatoploidy or aneuploidy. We tested whether a combination of chromosome counting, microsatellite analyses and flow cytometric measurements represents a suitable approach for the detection of basic chromosome numbers and ploidy in Kobresia (Cyperaceae). Chromosome counting resulted in 2n = 64 for Kobresia pygmaea and K. cercostachys, 2n = 58 and 64 for K. myosuroides, and 2n = 72 for K. simpliciuscula. We characterized eight microsatellite loci for K. pygmaea, which gave a maximum of four alleles per individual. Cross-species amplification was tested in 26 congeneric species and, on average, six of eight loci amplified successfully. Using flow cytometry, we confirmed tetraploidy in K. pygmaea. Basic chromosome numbers and ploidy were inferred from chromosome counts and the maximum number of alleles per locus. We consider the basic numbers as x = 16 and 18, with irregularities derived from agmatoploidy and aneuploidy. Across all Kobresia taxa, ploidy ranged from diploid up to heptaploid. The combination of chromosome counts and microsatellite analyses is an ideal method for the determination of basic chromosome numbers and for inferring ploidy, and flow cytometry is a suitable tool for the identification of deviating cytotypes. (C) 2014 The Linnean Society of London |
| 关键词 | 454 Sequencing Basic Chromosome Number Cross-amplification Kobresia Pygmaea Ecosystem Next-generation Sequencing Palaeopolyploidy Tibetan Plateau |
| 学科领域 | Plant Sciences |
| DOI | 10.1111/boj.12189 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341834900002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/18416 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 通讯作者 | Seeber,E (reprint author),Senckenberg Museum Nat Hist Goerlitz,Dept Bot,POB 300 154,D-02806 Goerlitz,Germany.; elke.seeber@senckenberg.de |
| 作者单位 | 1.Senckenberg Museum Nat Hist Goerlitz, Dept Bot, D-02806 Goerlitz, Germany 2.Univ Halle Wittenberg, Dept Geobot, D-06108 Halle, Saale, Germany 3.Univ Halle Wittenberg, Bot Garden, D-06108 Halle, Saale, Germany 4.German Ctr Integrat Biodivers Res iDiv, D-04103 Leipzig, Germany 5.Leibniz Inst Plant Genet & Crop Plant Res IPK, Apomixis Res Grp, D-06466 Stadt Seeland, Ot Gatersleben, Germany 6.UFZ Helmholtz Ctr Environm Res, Dept Community Ecol, D-06120 Halle, Saale, Germany 7.Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Gansu, Peoples R China 8.Chinese Acad Sci, Kunming Inst Bot, Heilongtan 650204, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Seeber, Elke,Winterfeld, Grit,Hensen, Isabell,et al. Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry[J]. BOTANICAL JOURNAL OF THE LINNEAN SOCIETY,2014,176(1):22-35. |
| APA | Seeber, Elke.,Winterfeld, Grit.,Hensen, Isabell.,Sharbel, Timothy F..,Durka, Walter.,...&elke.seeber@senckenberg.de.(2014).Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry.BOTANICAL JOURNAL OF THE LINNEAN SOCIETY,176(1),22-35. |
| MLA | Seeber, Elke,et al."Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry".BOTANICAL JOURNAL OF THE LINNEAN SOCIETY 176.1(2014):22-35. |
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