Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry
Seeber, Elke1; Winterfeld, Grit2,3; Hensen, Isabell2,3,4; Sharbel, Timothy F.5; Durka, Walter6; Liu, Jianquan7; Yang, Yong-Ping8; Wesche, Karsten1,4; Seeber,E (reprint author),Senckenberg Museum Nat Hist Goerlitz,Dept Bot,POB 300 154,D-02806 Goerlitz,Germany.; elke.seeber@senckenberg.de
2014-09-01
发表期刊BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
ISSN0024-4074
卷号176期号:1页码:22-35
产权排序第八
摘要Polyploidy is a fundamental mechanism in evolution, but is hard to detect in taxa with agmatoploidy or aneuploidy. We tested whether a combination of chromosome counting, microsatellite analyses and flow cytometric measurements represents a suitable approach for the detection of basic chromosome numbers and ploidy in Kobresia (Cyperaceae). Chromosome counting resulted in 2n = 64 for Kobresia pygmaea and K. cercostachys, 2n = 58 and 64 for K. myosuroides, and 2n = 72 for K. simpliciuscula. We characterized eight microsatellite loci for K. pygmaea, which gave a maximum of four alleles per individual. Cross-species amplification was tested in 26 congeneric species and, on average, six of eight loci amplified successfully. Using flow cytometry, we confirmed tetraploidy in K. pygmaea. Basic chromosome numbers and ploidy were inferred from chromosome counts and the maximum number of alleles per locus. We consider the basic numbers as x = 16 and 18, with irregularities derived from agmatoploidy and aneuploidy. Across all Kobresia taxa, ploidy ranged from diploid up to heptaploid. The combination of chromosome counts and microsatellite analyses is an ideal method for the determination of basic chromosome numbers and for inferring ploidy, and flow cytometry is a suitable tool for the identification of deviating cytotypes. (C) 2014 The Linnean Society of London
关键词454 Sequencing Basic Chromosome Number Cross-amplification Kobresia Pygmaea Ecosystem Next-generation Sequencing Palaeopolyploidy Tibetan Plateau
资助信息German Science Foundation as part of the DFG [SPP 1372, WE 2601/4-1, WE 2601/4-2]; Ministry of Science and Technology of China [2010DFB63500]
学科领域Plant Sciences
DOI10.1111/boj.12189
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000341834900002
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18416
专题资源植物与生物技术所级重点实验室
通讯作者Seeber,E (reprint author),Senckenberg Museum Nat Hist Goerlitz,Dept Bot,POB 300 154,D-02806 Goerlitz,Germany.; elke.seeber@senckenberg.de
作者单位1.Senckenberg Museum Nat Hist Goerlitz, Dept Bot, D-02806 Goerlitz, Germany
2.Univ Halle Wittenberg, Dept Geobot, D-06108 Halle, Saale, Germany
3.Univ Halle Wittenberg, Bot Garden, D-06108 Halle, Saale, Germany
4.German Ctr Integrat Biodivers Res iDiv, D-04103 Leipzig, Germany
5.Leibniz Inst Plant Genet & Crop Plant Res IPK, Apomixis Res Grp, D-06466 Stadt Seeland, Ot Gatersleben, Germany
6.UFZ Helmholtz Ctr Environm Res, Dept Community Ecol, D-06120 Halle, Saale, Germany
7.Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Gansu, Peoples R China
8.Chinese Acad Sci, Kunming Inst Bot, Heilongtan 650204, Kunming, Peoples R China
推荐引用方式
GB/T 7714
Seeber, Elke,Winterfeld, Grit,Hensen, Isabell,et al. Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry[J]. BOTANICAL JOURNAL OF THE LINNEAN SOCIETY,2014,176(1):22-35.
APA Seeber, Elke.,Winterfeld, Grit.,Hensen, Isabell.,Sharbel, Timothy F..,Durka, Walter.,...&elke.seeber@senckenberg.de.(2014).Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry.BOTANICAL JOURNAL OF THE LINNEAN SOCIETY,176(1),22-35.
MLA Seeber, Elke,et al."Ploidy in the alpine sedge Kobresia pygmaea (Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry".BOTANICAL JOURNAL OF THE LINNEAN SOCIETY 176.1(2014):22-35.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Seeber-2014-Ploidy i(327KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Seeber, Elke]的文章
[Winterfeld, Grit]的文章
[Hensen, Isabell]的文章
百度学术
百度学术中相似的文章
[Seeber, Elke]的文章
[Winterfeld, Grit]的文章
[Hensen, Isabell]的文章
必应学术
必应学术中相似的文章
[Seeber, Elke]的文章
[Winterfeld, Grit]的文章
[Hensen, Isabell]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。