Hanai,R (reprint author),Rikkyo Univ,Ctr Life Sci,Dept Life Sci & Res,Toshima Ku,Tokyo 1718501,Japan.
Two morphologically ambiguous Ligularia samples (samples A and B), and samples with morphology of Ligularia subspicata (sample C), Ligularia lamarum (sample D), or Ligularia cyathiceps (sample E), were collected at Tianchi Pond, Shangrila County, Yunnan Province, China. Analysis of the nucleotide sequence of the internal transcribed spacers (ITSs) in the nuclear ribosomal RNA gene cluster indicated that not only sample B but also sample D was a hybrid of L. cyathiceps and L. lamarum/ L. subspicata. Although the morphology of sample A suggested that it was also a hybrid, the ITS sequence of sample Awas that of L. cyathiceps. Twenty compounds were isolated from the five samples, and the structures of two new compounds 7 and 14 were determined. Furanoeremophilanes typical of L. lamarum/ L. subspicata were detected not only in samples C and D, but also in samples A and B. These results indicate that the ability to produce root chemicals can spread through introgression.
1.Rikkyo Univ, Ctr Smart Mol, Dept Chem & Res, Toshima Ku, Tokyo 1718501, Japan 2.Rikkyo Univ, Ctr Life Sci, Dept Life Sci & Res, Toshima Ku, Tokyo 1718501, Japan 3.Tokushima Bunri Univ, Fac Pharmaceut Sci, Yamashiro, Tokushima 7708514, Japan 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
Shimizu,Anna;Watanabe,Shizuka;Hanai,Ryo;Okamoto,Yasuko;Tori,Motoo;Gong,Xun;Kuroda,Chiaki.Chemical and Genetic Studies on Hybrid of Ligularia subspicata and Ligularia cyathiceps Collected in Yunnan Province of China,CHEMISTRY & BIODIVERSITY,2014,11(9):1438-1448