中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 植物化学与西部植物资源持续利用国家重点实验室  > 期刊论文
题名: A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study
作者: Xu, Jun1; Li, Song-Lin2, 3; Yue, Rui-Qi1; Ko, Chun-Hay4; Hu, Jiang-Miao5; Liu, Jing1; Ho, Hing-Man1; Yi, Tao1; Zhao, Zhong-Zhen1; Zhou, Jun5; Leung, Ping-Chung4; Chen, Hu-Biao1; Han, Quan-Bin1
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期: 2014-10-01
卷号: 406, 期号:25, 页码:6409-6417
关键词: HPGPC fingerprints ; Saccharide-dominant herbal materials ; Holistic polysaccharide marker ; Quality evaluation ; Dendrobium officinale
学科分类: Biochemistry & Molecular Biology ; Chemistry
DOI: 10.1007/s00216-014-8060-9
通讯作者: Chen,HB (reprint author),Hong Kong Baptist Univ,Sch Chinese Med,7 Baptist Univ Rd,Kowloon Tong,Hong Kong,Peoples R China. ; hbchen@hkbu.edu.hk ; simonhan@hkbu.edu.hk
文章类型: Article
英文摘要: Qualitative and quantitative characterization of natural saccharides, especially polysaccharides, in herb materials remains a challenge due to their complicated structures and high macromolecular masses. Currently available methods involve time-consuming and complicated operations, and present poor specificity. Here, a novel and rapid high-performance gel permeation chromatography (HPGPC)-based approach is described for quality assessment of saccharide-dominant herbal materials by simultaneous qualitative and quantitative analysis of saccharide components. Dendrobium officinale, one of the rarest tonic herbs worldwide, was employed as the model herb in this study. First, a HPGPC fingerprint based on the molecular weight distribution of its carbohydrate components was established for qualitative identification of D. officinale. Then, HPGPC-guided dominant holistic polysaccharide marker was separated using ultra-filtration followed by HPGPC determination for quantitative evaluation of D. officinale. The experimental results suggest that this method is more efficient, stable, and convenient compared with the currently available methods for authentication and quality evaluation of D. officinale, and we expect the method will have similar advantages when used for quality control of other saccharide-dominant herbal materials and products.
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: GAS-CHROMATOGRAPHY ; POLYSACCHARIDES ; SUGARS ; RADIX
收录类别: SCI
语种: 英语
WOS记录号: WOS:000342224300031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18380
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Xu-2014-A novel and rapid HP.pdf(841KB)----开放获取View Download

作者单位: 1.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China
2.China Acad ChineseMed Sci, Dept Pharmaceut Anal & Metab, Jiangsu Branch, Nanjing, Jiangsu, Peoples R China
3.Jiangsu Prov Acad Tradit Chinese Med, Nanjing, Jiangsu, Peoples R China
4.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Inst Chinese Med, Shatin, Hong Kong, Peoples R China
5.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming, Yunnan, Peoples R China

Recommended Citation:
Xu,Jun;Li,Song-Lin;Yue,Rui-Qi;Ko,Chun-Hay;Hu,Jiang-Miao;Liu,Jing;Ho,Hing-Man;Yi,Tao;Zhao,Zhong-Zhen;Zhou,Jun;Leung,Ping-Chung;Chen,Hu-Biao;Han,Quan-Bin.A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale, a case study,ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2014,406(25):6409-6417
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xu, Jun]'s Articles
[Li, Song-Lin]'s Articles
[Yue, Rui-Qi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xu, Jun]‘s Articles
[Li, Song-Lin]‘s Articles
[Yue, Rui-Qi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Xu-2014-A novel and rapid HP.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace