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Genomic imprinting, methylation and parent-of-origin effects in reciprocal hybrid endosperm of castor bean
Xu, Wei1,3; Dai, Mengyuan2,3; Li, Fei1; Liu, Aizhong1; Liu, AZ (reprint author), Chinese Acad Sci, Kunming Inst Bot, 132 Lanhei Rd, Kunming 650201, Peoples R China.; liuaizhong@mail.kib.ac.cn
2014
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume42Issue:11Pages:6987-6998
Contribution Rank第一
AbstractGenomic imprinting often results in parent-of-origin specific differential expression of maternally and paternally inherited alleles. In plants, the triploid endosperm is where gene imprinting occurs most often, but aside from studies on Arabidopsis, little is known about gene imprinting in dicotyledons. In this study, we inspected genomic imprinting in castor bean (Ricinus communis) endosperm, which persists throughout seed development. After mapping out the polymorphic SNP loci between accessions ZB306 and ZB107, we generated deep sequencing RNA profiles of F1 hybrid seeds derived from reciprocal crosses. Using polymorphic SNP sites to quantify allele-specific expression levels, we identified 209 genes in reciprocal endosperms with potential parent-of-origin specific expression, including 200 maternally expressed genes and 9 paternally expressed genes. In total, 57 of the imprinted genes were validated via reverse transcriptase-polymerase chain reaction sequencing, and analysis of the genomic DNA methylation distribution between embryo and endosperm tissues showed significant hypomethylation in the endosperm and an enrichment of differentially methylated regions around the identified genes. Curiously, the expression of the imprinted genes was not tightly linked to DNA methylation. These results largely extended gene imprinting information existing in plants, providing potential directions for further research in gene imprinting.
KeywordSeed-development Maize Endosperm Arabidopsis Endosperm Ricinus-communis Dna Methylation Gene-expression Demethylation Evolution Mammals Hypomethylation
Subject AreaBiochemistry & Molecular Biology
DOI10.1093/nar/gku375
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000338769400023
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18310
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorLiu, AZ (reprint author), Chinese Acad Sci, Kunming Inst Bot, 132 Lanhei Rd, Kunming 650201, Peoples R China.; liuaizhong@mail.kib.ac.cn
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Xu, Wei,Dai, Mengyuan,Li, Fei,et al. Genomic imprinting, methylation and parent-of-origin effects in reciprocal hybrid endosperm of castor bean[J]. NUCLEIC ACIDS RESEARCH,2014,42(11):6987-6998.
APA Xu, Wei,Dai, Mengyuan,Li, Fei,Liu, Aizhong,Liu, AZ ,&liuaizhong@mail.kib.ac.cn.(2014).Genomic imprinting, methylation and parent-of-origin effects in reciprocal hybrid endosperm of castor bean.NUCLEIC ACIDS RESEARCH,42(11),6987-6998.
MLA Xu, Wei,et al."Genomic imprinting, methylation and parent-of-origin effects in reciprocal hybrid endosperm of castor bean".NUCLEIC ACIDS RESEARCH 42.11(2014):6987-6998.
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