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题名: Genomic imprinting, methylation and parent-of-origin effects in reciprocal hybrid endosperm of castor bean
作者: Xu, Wei1, 3; Dai, Mengyuan2, 3; Li, Fei1; Liu, Aizhong1
刊名: NUCLEIC ACIDS RESEARCH
关键词: SEED-DEVELOPMENT ; MAIZE ENDOSPERM ; ARABIDOPSIS ENDOSPERM ; RICINUS-COMMUNIS ; DNA METHYLATION ; GENE-EXPRESSION ; DEMETHYLATION ; EVOLUTION ; MAMMALS ; HYPOMETHYLATION
英文摘要: Genomic imprinting often results in parent-of-origin specific differential expression of maternally and paternally inherited alleles. In plants, the triploid endosperm is where gene imprinting occurs most often, but aside from studies on Arabidopsis, little is known about gene imprinting in dicotyledons. In this study, we inspected genomic imprinting in castor bean (Ricinus communis) endosperm, which persists throughout seed development. After mapping out the polymorphic SNP loci between accessions ZB306 and ZB107, we generated deep sequencing RNA profiles of F1 hybrid seeds derived from reciprocal crosses. Using polymorphic SNP sites to quantify allele-specific expression levels, we identified 209 genes in reciprocal endosperms with potential parent-of-origin specific expression, including 200 maternally expressed genes and 9 paternally expressed genes. In total, 57 of the imprinted genes were validated via reverse transcriptase-polymerase chain reaction sequencing, and analysis of the genomic DNA methylation distribution between embryo and endosperm tissues showed significant hypomethylation in the endosperm and an enrichment of differentially methylated regions around the identified genes. Curiously, the expression of the imprinted genes was not tightly linked to DNA methylation. These results largely extended gene imprinting information existing in plants, providing potential directions for further research in gene imprinting.
出版日期: 2014
卷号: 42, 期号:11, 页码:6987-6998
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology
ISSN号: 0305-1048
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18310
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Key Lab Trop Plant Resource & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Xu,Wei;Dai,Mengyuan;Li,Fei;Liu,Aizhong.Genomic imprinting, methylation and parent-of-origin effects in reciprocal hybrid endosperm of castor bean,NUCLEIC ACIDS RESEARCH,2014,42(11):6987-6998
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