| Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea | |
Yang, Yunqiang1,2,3,5 ; Sun, Xudong2,3; Yang, Shihai4,5; Li, Xiong2,3,5; Yang, Yongping2,3; Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Biodivers & Biogeog,Kunming 650201,Peoples R China.; yangyp@mail.kib.ac.cn
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| 2014-05-30 | |
| 发表期刊 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| ISSN | 0006-291X |
| 卷号 | 448期号:2页码:145-150 |
| 摘要 | Stipa purpurea, an endemic forage of the grass family in the Tibetan Plateau, is highly resistant to drought. Dehydrins (DHNs) are stress proteins involved in plant protective reactions against environmental stress. In this study, the full-length DHN open reading frame (ORF) cloned from S. purpurea, named SpDHN1, was 816 nucleotides length and encoded a protein of 271 amino acids. Phylogenetic and sequence characterization analysis revealed that the DHN gene was an SK3-type DHNs. Subcellular localization analysis indicated that SpDHN1 was localized in the cytoplasm and the plasma membrane. SpDHN1 function analysis provided new evidence to support the antioxidation of SpDHN1 in plant responses drought stress. Ectopic expression of SpDHN1 in Arabidopsis thaliana plants showed more resistance to drought stress than the wild-type, indicating that SpDHN1 may be a potential candidate gene for genetic improvement of crops to improve stress tolerance. (C) 2014 Elsevier Inc. All rights reserved. |
| 关键词 | Stipa Purpurea Dehydrin Cloning Drought Antioxidation |
| 学科领域 | Biochemistry & Molecular Biology ; Biophysics |
| DOI | 10.1016/j.bbrc.2014.04.075 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336887000005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/18220 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 通讯作者 | Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Biodivers & Biogeog,Kunming 650201,Peoples R China.; yangyp@mail.kib.ac.cn |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog Easst Asia, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Kunming Inst Bot, Kunming 650201, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Peoples R China 4.Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing 100085, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yunqiang,Sun, Xudong,Yang, Shihai,et al. Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,448(2):145-150. |
| APA | Yang, Yunqiang.,Sun, Xudong.,Yang, Shihai.,Li, Xiong.,Yang, Yongping.,...&yangyp@mail.kib.ac.cn.(2014).Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,448(2),145-150. |
| MLA | Yang, Yunqiang,et al."Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 448.2(2014):145-150. |
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