Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea
Yang, Yunqiang1,2,3,5; Sun, Xudong2,3; Yang, Shihai4,5; Li, Xiong2,3,5; Yang, Yongping2,3; Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Biodivers & Biogeog,Kunming 650201,Peoples R China.; yangyp@mail.kib.ac.cn
2014-05-30
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
卷号448期号:2页码:145-150
摘要Stipa purpurea, an endemic forage of the grass family in the Tibetan Plateau, is highly resistant to drought. Dehydrins (DHNs) are stress proteins involved in plant protective reactions against environmental stress. In this study, the full-length DHN open reading frame (ORF) cloned from S. purpurea, named SpDHN1, was 816 nucleotides length and encoded a protein of 271 amino acids. Phylogenetic and sequence characterization analysis revealed that the DHN gene was an SK3-type DHNs. Subcellular localization analysis indicated that SpDHN1 was localized in the cytoplasm and the plasma membrane. SpDHN1 function analysis provided new evidence to support the antioxidation of SpDHN1 in plant responses drought stress. Ectopic expression of SpDHN1 in Arabidopsis thaliana plants showed more resistance to drought stress than the wild-type, indicating that SpDHN1 may be a potential candidate gene for genetic improvement of crops to improve stress tolerance. (C) 2014 Elsevier Inc. All rights reserved.
关键词Stipa Purpurea Dehydrin Cloning Drought Antioxidation
资助信息Major State Basic Research Development Program of China [2010CB951704];National Natural Science Foundation of China (NSFC) [41271058]
学科领域Biochemistry & Molecular Biology ; Biophysics
DOI10.1016/j.bbrc.2014.04.075
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000336887000005
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18220
专题资源植物与生物技术所级重点实验室
通讯作者Yang,YP (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Biodivers & Biogeog,Kunming 650201,Peoples R China.; yangyp@mail.kib.ac.cn
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog Easst Asia, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Kunming Inst Bot, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Peoples R China
4.Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing 100085, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Yang, Yunqiang,Sun, Xudong,Yang, Shihai,et al. Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,448(2):145-150.
APA Yang, Yunqiang.,Sun, Xudong.,Yang, Shihai.,Li, Xiong.,Yang, Yongping.,...&yangyp@mail.kib.ac.cn.(2014).Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,448(2),145-150.
MLA Yang, Yunqiang,et al."Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 448.2(2014):145-150.
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