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题名: Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis
作者: Xu, Ronghua1; Yang, Tianquan1, 2; Wang, Ruling1; Liu, Aizhong1, 3
刊名: FUNCTIONAL PLANT BIOLOGY
关键词: diacylglycerol acyltransferase ; fatty acid composition ; oil content ; triacylglycerols
英文摘要: Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants.
出版日期: 2014
卷号: 41, 期号:3, 页码:321-329
DOI标识: 10.1071/FP12388
语种: 英语
收录类别: SCI
学科分类: Plant Sciences
ISSN号: 1445-4408
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18219
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China

Recommended Citation:
Xu,Ronghua;Yang,Tianquan;Wang,Ruling;Liu,Aizhong.Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis,FUNCTIONAL PLANT BIOLOGY,2014,41(3):321-329
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