Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis
Xu, Ronghua1; Yang, Tianquan1,2; Wang, Ruling1; Liu, Aizhong1,3; Liu,AZ (reprint author),Chinese Acad Sci,Key Lab Trop Forest Ecol,Xishuangbanna Trop Bot Garden,88 Xuefu Rd,Kunming 650223,Peoples R China.; liuaizhong@mail.kib.ac.cn
2014
发表期刊FUNCTIONAL PLANT BIOLOGY
ISSN1445-4408
卷号41期号:3页码:321-329
摘要Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants.
关键词Diacylglycerol Acyltransferase Fatty Acid Composition Oil Content Triacylglycerols
资助信息NSFC [30871548, 31300569];'Hundred Talents Program of the Chinese Academy of Sciences'
学科领域Plant Sciences
DOI10.1071/FP12388
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000337368000012
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18219
专题资源植物与生物技术所级重点实验室
通讯作者Liu,AZ (reprint author),Chinese Acad Sci,Key Lab Trop Forest Ecol,Xishuangbanna Trop Bot Garden,88 Xuefu Rd,Kunming 650223,Peoples R China.; liuaizhong@mail.kib.ac.cn
作者单位1.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
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Xu, Ronghua,Yang, Tianquan,Wang, Ruling,et al. Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis[J]. FUNCTIONAL PLANT BIOLOGY,2014,41(3):321-329.
APA Xu, Ronghua,Yang, Tianquan,Wang, Ruling,Liu, Aizhong,Liu,AZ ,&liuaizhong@mail.kib.ac.cn.(2014).Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis.FUNCTIONAL PLANT BIOLOGY,41(3),321-329.
MLA Xu, Ronghua,et al."Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis".FUNCTIONAL PLANT BIOLOGY 41.3(2014):321-329.
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