| SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY | |
| Ping, Li1; Wang Yeling1; Guo Zhiyong1; Wu, Shibiao2,3; Kennelly, Edward J.1,2,3; Wang Jianjun1; Li, Tang1; Long Chunlin1,4; Long,CL (reprint author),Minzu Univ China,Coll Life & Environm Sci,27 Zhong Guan Cun South Ave,Beijing 100081,Peoples R China.; long@mail.kib.ac.cn | |
| 2014 | |
| 发表期刊 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| ISSN | 1082-6076 |
| 卷号 | 37期号:18页码:2598-2609 |
| 摘要 | Under LC-TOF-MS fraction-guided analysis, the n-butanol was extracted from Bidens pilosa. Three bioactivity isomers compounds, including methyl 3,4-dicaffeoyl quinate (1), methyl 3,5-dicaffeoyl quinate (2), and methyl 4,5-dicaffeoyl quinate (3) were successfully isolated by countercurrent chromatography (CCC) with two phase solvent system composed of chloroform-methanol-water-n-buthanol (4:3:2:0.5, v/v) elution. Their purities were 93.7%, 82.4%, and 91.8% determined by HPLC. HR-ESI-MS and NMR was used to confirm their structures. This is the first time the isolation of geometrical isomers of methyl dicaffeoyl quinates by CCC has been reported. |
| 关键词 | Bidens Pilosa l Bioactive Isomers Countercurrent Chromatography Lc-tof-ms Methyl Dicaffeoyl Quinate Separation |
| 学科领域 | Biochemical Research Methods ; Chemistry, Analytical |
| DOI | 10.1080/10826076.2013.853308 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337240500004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/18216 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 通讯作者 | Long,CL (reprint author),Minzu Univ China,Coll Life & Environm Sci,27 Zhong Guan Cun South Ave,Beijing 100081,Peoples R China.; long@mail.kib.ac.cn |
| 作者单位 | 1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China 2.CUNY Herbert H Lehman Coll, Dept Biol Sci, New York, NY USA 3.CUNY, Grad Ctr, New York, NY USA 4.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ping, Li,Wang Yeling,Guo Zhiyong,et al. SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(18):2598-2609. |
| APA | Ping, Li.,Wang Yeling.,Guo Zhiyong.,Wu, Shibiao.,Kennelly, Edward J..,...&long@mail.kib.ac.cn.(2014).SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(18),2598-2609. |
| MLA | Ping, Li,et al."SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.18(2014):2598-2609. |
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