SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY
Ping, Li1; Wang Yeling1; Guo Zhiyong1; Wu, Shibiao2,3; Kennelly, Edward J.1,2,3; Wang Jianjun1; Li, Tang1; Long Chunlin1,4; Long,CL (reprint author),Minzu Univ China,Coll Life & Environm Sci,27 Zhong Guan Cun South Ave,Beijing 100081,Peoples R China.; long@mail.kib.ac.cn
2014
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
卷号37期号:18页码:2598-2609
摘要Under LC-TOF-MS fraction-guided analysis, the n-butanol was extracted from Bidens pilosa. Three bioactivity isomers compounds, including methyl 3,4-dicaffeoyl quinate (1), methyl 3,5-dicaffeoyl quinate (2), and methyl 4,5-dicaffeoyl quinate (3) were successfully isolated by countercurrent chromatography (CCC) with two phase solvent system composed of chloroform-methanol-water-n-buthanol (4:3:2:0.5, v/v) elution. Their purities were 93.7%, 82.4%, and 91.8% determined by HPLC. HR-ESI-MS and NMR was used to confirm their structures. This is the first time the isolation of geometrical isomers of methyl dicaffeoyl quinates by CCC has been reported.
关键词Bidens Pilosa l Bioactive Isomers Countercurrent Chromatography Lc-tof-ms Methyl Dicaffeoyl Quinate Separation
资助信息National Natural Science Foundation of China [31070288, 31161140345];Ministry of Education of China [B08044, MUC 98506-01000101, MUC 98502-006004];Ministry of Science and Technology of China [2012FY110300-7];Fundamental Research Funds for the Central Universities [0910KYQN52]
学科领域Biochemical Research Methods ; Chemistry, Analytical
DOI10.1080/10826076.2013.853308
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000337240500004
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18216
专题资源植物与生物技术所级重点实验室
通讯作者Long,CL (reprint author),Minzu Univ China,Coll Life & Environm Sci,27 Zhong Guan Cun South Ave,Beijing 100081,Peoples R China.; long@mail.kib.ac.cn
作者单位1.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
2.CUNY Herbert H Lehman Coll, Dept Biol Sci, New York, NY USA
3.CUNY, Grad Ctr, New York, NY USA
4.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
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Ping, Li,Wang Yeling,Guo Zhiyong,et al. SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(18):2598-2609.
APA Ping, Li.,Wang Yeling.,Guo Zhiyong.,Wu, Shibiao.,Kennelly, Edward J..,...&long@mail.kib.ac.cn.(2014).SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(18),2598-2609.
MLA Ping, Li,et al."SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.18(2014):2598-2609.
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