Investigation of the Chemical Compositions in Tobacco of Different Origins and Maturities at Harvest by GC-MS and HPLC-PDA-QTOFMS
Xia, Bing1; Feng, Mengmeng1; Xu, Gang2; Xu, Jindi3; Li, Songlin3; Chen, Xiaozhen1; Ding, Lisheng1; Zhou, Yan1; Xu,G (reprint author),Chinese Acad Sci,Kunming Inst Bot,132 Lanhei Rd,Heilongtan 650204,Peoples R China.; xugang008@mail.kib.ac.cn; zhouyan@cib.ac.cn
2014-06-04
发表期刊JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
卷号62期号:22页码:4979-4987
摘要Tobacco samples of a same cultivar grown in different plantations in China were evaluated for their chemical compositions at different maturities for the first time. This was accomplished by a comprehensive and reliable method using gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography-photodiode array-quadrupole time-of-flight mass spectrometry (HPLC-PDA-QTOF-MS) to analyze the fat-soluble and polar components in 12 batches of tobacco samples of three origins and four maturities. The GC MS analyses showed that tobacco samples harvested at 40 days after transplantation exhibited more fat-soluble components, while those harvested at 100 days after transplantation exhibited the least fat-soluble components. Tentatively, identification of the main components as well as quantitative analyses of eight reference compounds, including five alkaloids, two polyphenols, and a coumarin, was performed by the developed HPLC-QTOF-PDA method. Results showed significant differences among origins and maturities in the contents of these compounds. The nicotine contents showed great variety among the 12 tobacco samples. The highest nicotine content were found in a sample from Zhengzhou harvested at 40 days after transplantation (ZZ-T with 25399.39 +/- 308.95 mu g/g), and the lowest nicotine level was detected in a sample from Zunyi harvested at 60 days after transplantation (ZY-X with 1654.49 +/- 34.52 mu g/g). The highest level of rutin was found in a Jiangchuan sample harvested at 60 days after transplantation (JC-X with 725.93 +/- 40.70 mu g/g), and the lowest rutin content was detected in a Zunyi tobacco sample harvested at 60 days after transplantation (ZY-X with 87.42 +/- 2.78 mu g/g). The developed method provided a convenient approach which might be applied for rapid maturity evaluation and tobacco flavor identification and also holds the potential for analysis of compounds present in other plants.
关键词Tobacco Chemical Compositions Maturities Planting Areas Hplc-qtof-pda-ms
资助信息National Natural Science Foundation of China [21302180];Major Science and Technology Program [110201101003-TS-03, 2011YN02, 2011YN03];Chinese Academy of Sciences
学科领域Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
DOI10.1021/jf5009204
收录类别SCI
语种英语
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
WOS类目Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号WOS:000337018700002
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/18198
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Xu,G (reprint author),Chinese Acad Sci,Kunming Inst Bot,132 Lanhei Rd,Heilongtan 650204,Peoples R China.; xugang008@mail.kib.ac.cn; zhouyan@cib.ac.cn
作者单位1.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu 610041, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Heilongtan 650204, Peoples R China
3.Jiangsu Prov Acad Tradit Chinese Med, Nanjing 210028, Jiangsu, Peoples R China
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GB/T 7714
Xia, Bing,Feng, Mengmeng,Xu, Gang,et al. Investigation of the Chemical Compositions in Tobacco of Different Origins and Maturities at Harvest by GC-MS and HPLC-PDA-QTOFMS[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(22):4979-4987.
APA Xia, Bing.,Feng, Mengmeng.,Xu, Gang.,Xu, Jindi.,Li, Songlin.,...&zhouyan@cib.ac.cn.(2014).Investigation of the Chemical Compositions in Tobacco of Different Origins and Maturities at Harvest by GC-MS and HPLC-PDA-QTOFMS.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(22),4979-4987.
MLA Xia, Bing,et al."Investigation of the Chemical Compositions in Tobacco of Different Origins and Maturities at Harvest by GC-MS and HPLC-PDA-QTOFMS".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.22(2014):4979-4987.
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