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题名: PLANT REGENERATION FROM LIQUID CALLUS CULTURES DERIVED FROM MATURE SEEDS OF PAMPAS GRASS [CORTADERIA SELLOANA (SCHULT. & SCHULT. F.) ASCH & GRAEBN.]
作者: Shimomae, Kazuki1; Niimi, Hiroyuki1; Chin, Dong Poh1; Khan, Raham Sher1, 2; Lu, Yuanxue3; Ogawa, Yoichi4; Mii, Masahiro1, 4
刊名: PROPAGATION OF ORNAMENTAL PLANTS
关键词: micropropagation ; ornamental grass ; picloram
英文摘要: An efficient system of plant regeneration from liquid culture of mature seed-derived callus was established in pampas grass [Cortaderia selloana (Schult. & Schult.f.) Asch. & Graebn.]. Calluses were induced from mature seeds on MS medium containing 30 g l(-1) maltose, 8 g l(-1) agar, and different concentrations (0, 1, 2, and 5 mg l(-1)) of dicamba, NAA, picloram or 2,4-D. The highest callus induction was obtained on medium containing 5 mg l(-1) picloram where 98% explants formed callus. Calluses were subcultured on MS medium containing 2 mg l(-1) dicamba, NAA, picloram or 2,4-D and 30 g l(-1) maltose for 3 months and the compact calluses obtained were transferred into the same corresponding liquid media. By using 2 month-old liquid-cultured calluses, effects of different concentrations of BAP (0, 0.1, 0.5, and 1 mg l(-1)) and NAA (0, 0.1, 0.5, and 1 mg l(-1)) on shoot regeneration were evaluated using MS medium with 30 g l(-1) sucrose, 8 g l(-1) agar, and 0.5 mg l(-1) GA(3) supplemented to the basal medium. The highest frequency of shoot formation was obtained in callus induced by picloram on medium containing 0.5 mg l(-1) BAP and 0.5 mg(-1) NAA, where 92% of the calluses formed shoots. The calluses maintained high shoot regeneration ability (71-85%) and original ploidy level of diploid during the 8 months of subcultures, although drastic reduction in the regeneration ability (< 23%) and the appearance of tetraploid cells were observed after 12 months of subculture in liquid media.
出版日期: 2014-03-01
卷号: 14, 期号:1, 页码:20-25
语种: 英语
ISSN号: 1311-9109
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/18060
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chiba Univ, Grad Sch Hort, Lab Plant Cell Technol, Matsudo, Chiba 2718510, Japan
2.Abdul Wali Khan Univ Mardan, Dept Biotechnol, Mardan, Pakistan
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650204, Yunnan, Peoples R China
4.Chiba Univ, Ctr Environm Hlth & Field Sci, Kashiwa, Chiba 2770882, Japan

Recommended Citation:
Shimomae, Kazuki,Niimi, Hiroyuki,Chin, Dong Poh,et al. PLANT REGENERATION FROM LIQUID CALLUS CULTURES DERIVED FROM MATURE SEEDS OF PAMPAS GRASS [CORTADERIA SELLOANA (SCHULT. & SCHULT. F.) ASCH & GRAEBN.][J]. PROPAGATION OF ORNAMENTAL PLANTS,2014,14(1):20-25.
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