The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells
Cheng, Lin1; Xia, Tian-Song1; Wang, Yi-Fen2; Zhou, Wenbin1; Liang, Xiu-Qing1; Xue, Jin-Qiu1; Shi, Liang1; Wang, Ying1; Ding, Qiang1
Corresponding AuthorDing, Q (reprint author), Nanjing Med Univ, Affiliated Hosp 1, Jiangsu Breast Dis Ctr, Nanjing, Jiangsu, Peoples R China. ; dingqiang@njmu.edu.cn
2014-03-06
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:3Pages:e90848
AbstractThere is growing interest in development of natural products as anti-cancer and chemopreventive agents. Many triterpenoids have been proved as potential agents for chemoprevention and therapy of breast cancer. Ginsenosides from ginseng, which mostly belong to dammarane-type triterpenoids, have gained great attention for their anti-breast cancer activity with diverse mechanisms. However, studies of other kinds of triterpenoid saponins on breast cancer are limited. Previously, we purified and identified a novel oleanane-type triterpene saponin named D Rhamnose beta-hederin (DR beta-H) from Clematis ganpiniana, a Chinese traditional anti-tumor herb. In the present study, DR beta-H showed strong inhibitory activity on the growth of various breast cancer cells and induced apoptosis in these cells. DR beta-H inhibited PI3K/AKT and activated ERK signaling pathway. PI3K inhibitor LY294002 synergistically enhanced DR beta-H-induced apoptosis whereas MEK inhibitor U0126 reduced the apoptosis rate. Moreover, DR beta-H regulated the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins. Furthermore, DR beta-H induced depolarization of mitochondrial membrane potential which released Apaf-1 and Cytochrome C from the inter membrane space into the cytosol, where they promoted caspase-9 and caspase-3 activation. This is the first report on the pro-apoptotic effects of DR beta-H, a novel oleanane-type triterpenoid saponin, on breast cancer cells and its comprehensive apoptosis pathways. It implied that oleanane-type triterpenoid saponin DR beta-H could be a promising candidate for chemotherapy of breast cancer.
KeywordOxygen Species Generation Bcl-2 Family Proteins Platycodon-grandiflorum Ginsenoside Rh2 Hepatoma-cells Mapk Pathways Anticancer Activation Induction Death
Subject AreaMultidisciplinary Sciences
DOI10.1371/journal.pone.0090848
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000332483600095
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/18051
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Nanjing Med Univ, Affiliated Hosp 1, Jiangsu Breast Dis Ctr, Nanjing, Jiangsu, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Lin,Xia, Tian-Song,Wang, Yi-Fen,et al. The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells[J]. PLOS ONE,2014,9(3):e90848.
APA Cheng, Lin.,Xia, Tian-Song.,Wang, Yi-Fen.,Zhou, Wenbin.,Liang, Xiu-Qing.,...&Ding, Qiang.(2014).The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells.PLOS ONE,9(3),e90848.
MLA Cheng, Lin,et al."The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells".PLOS ONE 9.3(2014):e90848.
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