KIB OpenIR  > 资源植物与生物技术所级重点实验室
Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells
Zhang, Nan1; Han, Zhentai2; Sun, Guiling3; Hoffman, Angela4; Wilson, Iain W.5; Yang, Yanfang1; Gao, Qian2; Wu, Jianqiang3; Xie, Dan6,7; Dai, Jungui6,7; Qiu, Deyou1
Corresponding AuthorQiu,DY (reprint author),Chinese Acad Forestry,State Key Lab Tree Genet & Breeding,Res Inst Forestry,Beijing 100091,Peoples R China. ; qiudy@caf.ac.cn
2014-01-17
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume443Issue:3Pages:938-943
AbstractTaxol is a well-known effective anticancer compound. Due to the inability to synthesize sufficient quantities of taxol to satisfy commercial demand, a biotechnological approach for a large-scale cell or cell-free system for its production is highly desirable. Several important genes in taxol biosynthesis are currently still unknown and have been shown to be difficult to isolate directly from Taxus, including the gene encoding taxoid 9 alpha-hydroxylase. Ginkgo biloba suspension cells exhibit taxoid hydroxylation activity and provides an alternate means of identifying genes encoding enzymes with taxoid 9 alpha-hydroxylation activity. Through analysis of high throughput RNA sequencing data from G. biloba, we identified two candidate genes with high similarity to Taxus CYP450s. Using in vitro cell-free protein synthesis assays and LC-MS analysis, we show that one candidate that belongs to the CYP716B, a subfamily whose biochemical functions have not been previously studied, possessed 9 alpha-hydroxylation activity. This work will aid future identification of the taxoid 9 alpha-hydroxylase gene from Taxus sp. (C) 2013 Elsevier Inc. All rights reserved.
KeywordTaxol Taxoid 9 Alpha-hydroxylase Ginkgo Biloba Cyp450 Cell-free Protein Synthesis System Lc-ms
Subject AreaBiochemistry & Molecular Biology ; Biophysics
DOI10.1016/j.bbrc.2013.12.104
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000331415000026
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/17983
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China
2.Chinese Acad Forestry, Key Lab Forest Ecol Environm, Minist Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China
3.Chinese Acad Sci, Key Lab Econ Plants & Biotechnol, Kunming Inst Bot, Kunming 650201, Peoples R China
4.Univ Portland, Dept Chem, Portland, OR 97203 USA
5.CSIRO Plant Ind, Canberra, ACT 2001, Australia
6.Chinese Acad Med Sci, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing 100050, Peoples R China
7.Peking Union Med Coll, Beijing 100050, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Nan,Han, Zhentai,Sun, Guiling,et al. Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,443(3):938-943.
APA Zhang, Nan.,Han, Zhentai.,Sun, Guiling.,Hoffman, Angela.,Wilson, Iain W..,...&Qiu, Deyou.(2014).Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,443(3),938-943.
MLA Zhang, Nan,et al."Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 443.3(2014):938-943.
Files in This Item: Download All
File Name/Size DocType Version Access License
Zhang-2014-Molecular(440KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Nan]'s Articles
[Han, Zhentai]'s Articles
[Sun, Guiling]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Nan]'s Articles
[Han, Zhentai]'s Articles
[Sun, Guiling]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Nan]'s Articles
[Han, Zhentai]'s Articles
[Sun, Guiling]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Zhang-2014-Molecular cloning an.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.