Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells
Zhang, Nan1; Han, Zhentai2; Sun, Guiling3; Hoffman, Angela4; Wilson, Iain W.5; Yang, Yanfang1; Gao, Qian2; Wu, Jianqiang3; Xie, Dan6,7; Dai, Jungui6,7; Qiu, Deyou1
2014-01-17
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
卷号443期号:3页码:938-943
摘要Taxol is a well-known effective anticancer compound. Due to the inability to synthesize sufficient quantities of taxol to satisfy commercial demand, a biotechnological approach for a large-scale cell or cell-free system for its production is highly desirable. Several important genes in taxol biosynthesis are currently still unknown and have been shown to be difficult to isolate directly from Taxus, including the gene encoding taxoid 9 alpha-hydroxylase. Ginkgo biloba suspension cells exhibit taxoid hydroxylation activity and provides an alternate means of identifying genes encoding enzymes with taxoid 9 alpha-hydroxylation activity. Through analysis of high throughput RNA sequencing data from G. biloba, we identified two candidate genes with high similarity to Taxus CYP450s. Using in vitro cell-free protein synthesis assays and LC-MS analysis, we show that one candidate that belongs to the CYP716B, a subfamily whose biochemical functions have not been previously studied, possessed 9 alpha-hydroxylation activity. This work will aid future identification of the taxoid 9 alpha-hydroxylase gene from Taxus sp. (C) 2013 Elsevier Inc. All rights reserved.
关键词Taxol Taxoid 9 Alpha-hydroxylase Ginkgo Biloba Cyp450 Cell-free Protein Synthesis System Lc-ms
资助信息Chinese Academy of Forestry [CAFYBB2012042]
学科领域Biochemistry & Molecular Biology ; Biophysics
DOI10.1016/j.bbrc.2013.12.104
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000331415000026
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17983
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China
2.Chinese Acad Forestry, Key Lab Forest Ecol Environm, Minist Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China
3.Chinese Acad Sci, Key Lab Econ Plants & Biotechnol, Kunming Inst Bot, Kunming 650201, Peoples R China
4.Univ Portland, Dept Chem, Portland, OR 97203 USA
5.CSIRO Plant Ind, Canberra, ACT 2001, Australia
6.Chinese Acad Med Sci, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing 100050, Peoples R China
7.Peking Union Med Coll, Beijing 100050, Peoples R China
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Zhang, Nan,Han, Zhentai,Sun, Guiling,et al. Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,443(3):938-943.
APA Zhang, Nan.,Han, Zhentai.,Sun, Guiling.,Hoffman, Angela.,Wilson, Iain W..,...&Qiu, Deyou.(2014).Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,443(3),938-943.
MLA Zhang, Nan,et al."Molecular cloning and characterization of a cytochrome P450 taxoid 9a-hydroxylase in Ginkgo biloba cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 443.3(2014):938-943.
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