GLUT4 is Sorted into Vesicles Whose Accumulation Beneath and Insertion to the Plasma Membrane is Differentially Regulated by Insulin and Selectively by Insulin Resistance
Wenyong Xiong ; Ingrid Jordens ; Eva Gonzalez ; Timothy McGraw
2010
Source PublicationMolecular Biology of the Cell
Volume21Issue:8Pages:1375-1386
AbstractInsulin stimulates glucose transport by recruiting the GLUT4 glucose transporter to the plasma membrane. Here we use total internal reflection fluorescence microscopy to show that two trafficking motifs of GLUT4, a FQQI motif and a TELE-based motif, target GLUT4 to specialized vesicles that accumulate adjacent to the plasma membrane of unstimulated adipocytes. Mutations of these motifs redistributed GLUT4 to transferrin-containing recycling vesicles adjacent to the plasma membrane, and the degree of redistribution correlated with the increases of the GLUT4 mutants in the plasma membrane of basal adipocytes. These results establish that GLUT4 defaults to recycling endosomes when trafficking to specialized vesicles is disrupted, supporting the hypothesis that the specialized vesicles are derived from an endosomal compartment. Insulin stimulates both the accumulation of GLUT4 in the evanescent field and the fraction of this GLUT4 that is inserted into the plasma membrane. Unexpectedly, these two steps are differentially affected by the development of insulin resistance. We ascribe this selective insulin resistance to inherent differences in the sensitivities of GLUT4 vesicle accumulation and insertion into the plasma membrane to insulin. Differences in insulin sensitivities of various processes may be a general mechanism for the development of the physiologically important phenomenon of selective insulin resistance.
Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/17639
Collection植物化学与西部植物资源持续利用国家重点实验室
Recommended Citation
GB/T 7714
Wenyong Xiong,Ingrid Jordens,Eva Gonzalez,et al. GLUT4 is Sorted into Vesicles Whose Accumulation Beneath and Insertion to the Plasma Membrane is Differentially Regulated by Insulin and Selectively by Insulin Resistance[J]. Molecular Biology of the Cell,2010,21(8):1375-1386.
APA Wenyong Xiong,Ingrid Jordens,Eva Gonzalez,&Timothy McGraw.(2010).GLUT4 is Sorted into Vesicles Whose Accumulation Beneath and Insertion to the Plasma Membrane is Differentially Regulated by Insulin and Selectively by Insulin Resistance.Molecular Biology of the Cell,21(8),1375-1386.
MLA Wenyong Xiong,et al."GLUT4 is Sorted into Vesicles Whose Accumulation Beneath and Insertion to the Plasma Membrane is Differentially Regulated by Insulin and Selectively by Insulin Resistance".Molecular Biology of the Cell 21.8(2010):1375-1386.
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