Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum

doi:10.1055/s-0031-1298431

	Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum
	Dzoyem, Jean P.1; NKuete, Antoine H. L.2; Kuete, Victor 1; Tala, Michel F.2; Wabo, Hippolyte K.2; Guru, Santosh K.3; Rajput, Vikrant S.4; Sharma, Akash 4; Tane, Pierre 2; Khan, Inshad A.4; Saxena, Anil K.4; Laatsch, Hartmut 5; Tan, Ning-Hua 6
通讯作者	Kuete, V (reprint author), Univ Dschang, Fac Sci, Dept Biochem, POB 67, Dschang 237, Cameroon,kuetevictor@yahoo.fr
	2012-05-01
发表期刊	PLANTA MEDICA
ISSN	0032-0943
卷号	78 期号:8 页码:787-792
摘要	The present study was designed to investigate the antimicrobial activity and the cytotoxicity of the methanol extract (PLA) as well as fractions (PLA1-4) and compounds [cardamomin (1), (+/-)-polygohomoisoflavanone (2), (S)-(-)-pinostrobin (3), 2',4'-dihydroxy-3',6'-dimethoxychalcone (4), (2S)-(-)-5-hydroxy-6,7-dimethoxyflavanone (5), and (2S)-(-)-5,7-dimethoxyflavanone (6)] obtained from leaves of Polygonum limbatum. The microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against 11 microbial strains including Candida albicans, C. krusei, C. tropicalis, Aspergillus fumigatus, Pseudomonas aeruginosa, Escherichia coli, vancomycin-resistant Enterococcus faecalis (VRE), Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S. epidermidis, and Mycobacterium tuberculosis H37Rv. The sulphorhodamine B cell growth inhibition assay was used to assess the cytotoxicity of the above samples on lung A549 adenocarcinoma, breast carcinoma MCF-7, prostate carcinoma PC-3, cervical carcinoma HeLa, and the acute monocytic leukemia cell line THP-1. The results of the MIC determination indicated that, apart from fraction PLA3, all other fractions as well as PLA and compound 3 were selectively active. MIC values were noted on 100% of the 11 tested microorganisms for fraction PLA3, 72.7% for PLA, fraction PLA2, and compound 4, 63.6% for PLA1, and 54.5% for fraction PLA4. The results of the cytotoxicity assay revealed that, except for A459 cells, more than 50% inhibition of the proliferation was obtained with each of the tested samples on at least one of the four other cell lines. IC50 values below 4 mu g/mL were obtained with 1 and 4 on THP-1 cells. The overall results of the present study provided baseline information for the possible use of Polygonum limbatum as well as some of the isolated compounds for the control of cancer diseases and mostly leukemia.
关键词	Polygonum Limbatum Polygonaceae Extract Compounds Antimicrobial Cytotoxicity
学科领域	Plant Sciences ; Pharmacology & Pharmacy
DOI	10.1055/s-0031-1298431
收录类别	SCI
语种	英语
WOS记录号	WOS:000304449300007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/17621
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Univ Dschang, Fac Sci, Dept Biochem, Dschang 237, Cameroon 2.Univ Dschang, Fac Sci, Dept Chem, Dschang 237, Cameroon 3.Indian Inst Integrat Med, Canc Pharmacol Div, Cell Signalling Lab, Jammu, India 4.Indian Inst Integrat Med, Clin Microbiol Unit, Jammu, India 5.Univ Gottingen, Inst Organ & Biomol Chem, Gottingen, Germany 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Dzoyem, Jean P.,NKuete, Antoine H. L.,Kuete, Victor,et al. Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum[J]. PLANTA MEDICA,2012,78(8):787-792.
APA	Dzoyem, Jean P..,NKuete, Antoine H. L..,Kuete, Victor.,Tala, Michel F..,Wabo, Hippolyte K..,...&Tan, Ning-Hua.(2012).Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum.PLANTA MEDICA,78(8),787-792.
MLA	Dzoyem, Jean P.,et al."Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum".PLANTA MEDICA 78.8(2012):787-792.

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