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Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation
Liu, Hui1,2; Wang, Cuiping1,2; Komatsu, Setsuko3; He, Mingxia4; Liu, Gongshe1; Shen, Shihua1
Corresponding AuthorLiu,GS (reprint author),Chinese Acad Sci,Inst Bot,Key Lab Res & Dev Resource Plant,Beijing 100093,Peoples R China., ;
AbstractTo characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to Profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25 days after flowering at the late stage of seed development of J. curcas. Furthermore, C-16:0 was initially synthesized as the precursor for the elongation to C-18:1 and C-18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas.
KeywordJatropha Curcas Proteomics Seed Development Carbon Flux Lipid Accumulation
Subject AreaBiochemistry & Molecular Biology
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000327906000003
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,et al. Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation[J]. JOURNAL OF PROTEOMICS,2013,91:23-40.
APA Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,He, Mingxia,Liu, Gongshe,&Shen, Shihua.(2013).Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation.JOURNAL OF PROTEOMICS,91,23-40.
MLA Liu, Hui,et al."Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation".JOURNAL OF PROTEOMICS 91(2013):23-40.
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