KIB OpenIR  > 资源植物与生物技术所级重点实验室
Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation
Liu, Hui1,2; Wang, Cuiping1,2; Komatsu, Setsuko3; He, Mingxia4; Liu, Gongshe1; Shen, Shihua1
Corresponding AuthorLiu,GS (reprint author),Chinese Acad Sci,Inst Bot,Key Lab Res & Dev Resource Plant,Beijing 100093,Peoples R China.,liugs@ibcas.ac.cn ; shshen@ibcas.ac.cn
2013-10-08
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume91Pages:23-40
AbstractTo characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to Profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25 days after flowering at the late stage of seed development of J. curcas. Furthermore, C-16:0 was initially synthesized as the precursor for the elongation to C-18:1 and C-18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas.
KeywordJatropha Curcas Proteomics Seed Development Carbon Flux Lipid Accumulation
Subject AreaBiochemistry & Molecular Biology
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000327906000003
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/17437
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,et al. Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation[J]. JOURNAL OF PROTEOMICS,2013,91:23-40.
APA Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,He, Mingxia,Liu, Gongshe,&Shen, Shihua.(2013).Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation.JOURNAL OF PROTEOMICS,91,23-40.
MLA Liu, Hui,et al."Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation".JOURNAL OF PROTEOMICS 91(2013):23-40.
Files in This Item: Download All
File Name/Size DocType Version Access License
Liu-2013-Proteomic a(2034KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Hui]'s Articles
[Wang, Cuiping]'s Articles
[Komatsu, Setsuko]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Hui]'s Articles
[Wang, Cuiping]'s Articles
[Komatsu, Setsuko]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Hui]'s Articles
[Wang, Cuiping]'s Articles
[Komatsu, Setsuko]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Liu-2013-Proteomic analysis o.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.