Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation
Liu, Hui1,2; Wang, Cuiping1,2; Komatsu, Setsuko3; He, Mingxia4; Liu, Gongshe1; Shen, Shihua1
2013-10-08
发表期刊JOURNAL OF PROTEOMICS
ISSN1874-3919
卷号91页码:23-40
摘要To characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to Profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25 days after flowering at the late stage of seed development of J. curcas. Furthermore, C-16:0 was initially synthesized as the precursor for the elongation to C-18:1 and C-18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas.
关键词Jatropha Curcas Proteomics Seed Development Carbon Flux Lipid Accumulation
资助信息National Natural Science Foundation of China [31270653];Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-YW-G-035]
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/17437
专题资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan
4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
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Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,et al. Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation[J]. JOURNAL OF PROTEOMICS,2013,91:23-40.
APA Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,He, Mingxia,Liu, Gongshe,&Shen, Shihua.(2013).Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation.JOURNAL OF PROTEOMICS,91,23-40.
MLA Liu, Hui,et al."Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation".JOURNAL OF PROTEOMICS 91(2013):23-40.
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