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题名: Novel Inhibitor Cystine Knot Peptides from Momordica charantia
作者: He, Wen-Jun1; Chan, Lai Yue2; Clark, Richard J.2, 3; Tang, Jun1; Zeng, Guang-Zhi1; Franco, Octavio L.4; Cantacessi, Cinzia5; Craik, David J.2; Daly, Norelle L.2, 5; null(谭宁华)1
刊名: PLOS ONE
关键词: AMINO-ACID-SEQUENCES ; TRYPSIN-INHIBITOR ; PLASMODIUM-FALCIPARUM ; PROTEINASE-INHIBITORS ; CYTOTOXIC CYCLOTIDES ; MASS-SPECTROMETRY ; FOLDING PATHWAYS ; LINN SEEDS ; KALATA B1 ; X-RAY
英文摘要: Two new peptides, MCh-1 and MCh-2, along with three known trypsin inhibitors (MCTI-I, MCTI-II and MCTI-III), were isolated from the seeds of the tropical vine Momordica charantia. The sequences of the peptides were determined using mass spectrometry and NMR spectroscopy. Using a strategy involving partial reduction and stepwise alkylation of the peptides, followed by enzymatic digestion and tandem mass spectrometry sequencing, the disulfide connectivity of MCh-1 was elucidated to be CysI-CysIV, CysII-CysV and CysIII-CysVI. The three-dimensional structures of MCh-1 and MCh-2 were determined using NMR spectroscopy and found to contain the inhibitor cystine knot (ICK) motif. The sequences of the novel peptides differ significantly from peptides previously isolated from this plant. Therefore, this study expands the known peptide diversity in M. charantia and the range of sequences that can be accommodated by the ICK motif. Furthermore, we show that a stable two-disulfide intermediate is involved in the oxidative folding of MCh-1. This disulfide intermediate is structurally homologous to the proposed ancestral fold of ICK peptides, and provides a possible pathway for the evolution of this structural motif, which is highly prevalent in nature.
出版日期: 2013-10-08
卷号: 8, 期号:10, 页码:e75334
语种: 英语
收录类别: SCI
学科分类: Multidisciplinary Sciences
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/17278
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia
3.Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia
4.Univ Catolica Brasilia, Ctr Anal Prote & Bioquim, Programa Posgrad Ciencias Genom & Biotecnol, Brasilia, DF, Brazil
5.James Cook Univ, Ctr Biodiscovery & Mol Dev Therapeut, Cairns, Qld, Australia

Recommended Citation:
He, Wen-Jun;Chan, Lai Yue;Clark, Richard J.;Tang, Jun;Zeng, Guang-Zhi;Franco, Octavio L.;Cantacessi, Cinzia;Craik, David J.;Daly, Norelle L.;Tan, Ning-Hua.Novel Inhibitor Cystine Knot Peptides from Momordica charantia,PLOS ONE,2013,8(10):e75334
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