Novel Inhibitor Cystine Knot Peptides from Momordica charantia

	Novel Inhibitor Cystine Knot Peptides from Momordica charantia
	He, Wen-Jun 1; Chan, Lai Yue 2; Clark, Richard J.2,3; Tang, Jun 1; Zeng, Guang-Zhi 1; Franco, Octavio L.4; Cantacessi, Cinzia 5; Craik, David J.2; Daly, Norelle L.2,5; Tan, Ning-Hua 1
通讯作者	Daly, NL (reprint author), Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia.,norelle.daly@jcu.edu.au ; nhtan@mail.kib.ac.cn
	2013-10-08
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:10 页码:e75334
摘要	Two new peptides, MCh-1 and MCh-2, along with three known trypsin inhibitors (MCTI-I, MCTI-II and MCTI-III), were isolated from the seeds of the tropical vine Momordica charantia. The sequences of the peptides were determined using mass spectrometry and NMR spectroscopy. Using a strategy involving partial reduction and stepwise alkylation of the peptides, followed by enzymatic digestion and tandem mass spectrometry sequencing, the disulfide connectivity of MCh-1 was elucidated to be CysI-CysIV, CysII-CysV and CysIII-CysVI. The three-dimensional structures of MCh-1 and MCh-2 were determined using NMR spectroscopy and found to contain the inhibitor cystine knot (ICK) motif. The sequences of the novel peptides differ significantly from peptides previously isolated from this plant. Therefore, this study expands the known peptide diversity in M. charantia and the range of sequences that can be accommodated by the ICK motif. Furthermore, we show that a stable two-disulfide intermediate is involved in the oxidative folding of MCh-1. This disulfide intermediate is structurally homologous to the proposed ancestral fold of ICK peptides, and provides a possible pathway for the evolution of this structural motif, which is highly prevalent in nature.
关键词	Amino-acid-sequences Trypsin-inhibitor Plasmodium-falciparum Proteinase-inhibitors Cytotoxic Cyclotides Mass-spectrometry Folding Pathways Linn Seeds Kalata B1 X-ray
学科领域	Multidisciplinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000325552200016
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/17278
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming, Yunnan, Peoples R China 2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia 3.Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia 4.Univ Catolica Brasilia, Ctr Anal Prote & Bioquim, Programa Posgrad Ciencias Genom & Biotecnol, Brasilia, DF, Brazil 5.James Cook Univ, Ctr Biodiscovery & Mol Dev Therapeut, Cairns, Qld, Australia
推荐引用方式 GB/T 7714	He, Wen-Jun,Chan, Lai Yue,Clark, Richard J.,et al. Novel Inhibitor Cystine Knot Peptides from Momordica charantia[J]. PLOS ONE,2013,8(10):e75334.
APA	He, Wen-Jun.,Chan, Lai Yue.,Clark, Richard J..,Tang, Jun.,Zeng, Guang-Zhi.,...&Tan, Ning-Hua.(2013).Novel Inhibitor Cystine Knot Peptides from Momordica charantia.PLOS ONE,8(10),e75334.
MLA	He, Wen-Jun,et al."Novel Inhibitor Cystine Knot Peptides from Momordica charantia".PLOS ONE 8.10(2013):e75334.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
He-2013-Novel Inhibi（1766KB）			开放获取	CC BY-NC-SA	浏览下载