Amide N-glycosylation by Asm25, an N-glycosyltransferase of ansamitocins
Zhao, Peiji2; Bai, Linquan1; Ma, Juan2; Zeng, Ying2; Li, Lei1; Zhang, Yirong1; Lu, Chunhua3; Dai, Huanqin2; Wu, Zhaoxian3; Li, Yaoyao3; Wu, Xuan3; Chen, Gang2; Hao, Xiaojiang2; Shen, Yuemao2,3; Deng, Zixin1; Floss, Heinz G.4
2008-08-25
发表期刊CHEMISTRY & BIOLOGY
ISSN1074-5521
卷号15期号:8页码:863-874
摘要Ansamitocins are potent antitumor maytansinoids produced by Actinosynnema pretiosum. Their biosynthesis involves the initial assembly of a macrolactam polyketide, followed by a series of postpolyketide synthase (PKS) modifications. Three ansamitocin glycosides were isolated from A. pretiosum and fully characterized structurally as novel ansamitocin derivatives, carrying a (3-D-glucosyl group attached to the macrolactam amide nitrogen in place of the N-methyl group. By gene inactivation and complementation, asm25 was identified as the N-glycosyltransferase gene responsible for the macrolactam amide N-glycosylation of ansamitocins. Soluble, enzymatically active Asm25 protein was obtained from asm25-expressing E, coli by solubilization from inclusion bodies. Its optimal reaction conditions, including temperature, pH, metal ion requirement, and Km/Kcat, were determined. Asm25 also showed broad substrate specificity toward other ansamycins and synthetic indolin-2-ones. To the best of our knowledge, this represents the first in vitro characterization of a purified antibiotic N-glycosyltransferase.
关键词Biosynthetic Gene-cluster Lechevalieria-aerocolonigenes Atcc-39243 Escherichia-coli 3-amino-5-hydroxybenzoic Acid Actinosynnema-pretiosum Maytansinoid Antibiotics Streptomyces Sp Indolocarbazole Expression Substrate
资助信息National Natural Science Fund for Distinguished Young Scholars [30325044]; Natural Science Foundation of China [30600005, 30570019]; Ministry of Science and Technology
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/1707
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Lab Microbial Metab, Shanghai 200240, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan, Peoples R China
3.Xiamen Univ, Key Lab Minist Educ Cell Biol & Tumor Cell Engn, Sch Life Sci, Fujian 361005, Peoples R China
4.Univ Washington, Dept Chem, Seattle, WA 98195 USA
推荐引用方式
GB/T 7714
Zhao, Peiji,Bai, Linquan,Ma, Juan,et al. Amide N-glycosylation by Asm25, an N-glycosyltransferase of ansamitocins[J]. CHEMISTRY & BIOLOGY,2008,15(8):863-874.
APA Zhao, Peiji.,Bai, Linquan.,Ma, Juan.,Zeng, Ying.,Li, Lei.,...&Floss, Heinz G..(2008).Amide N-glycosylation by Asm25, an N-glycosyltransferase of ansamitocins.CHEMISTRY & BIOLOGY,15(8),863-874.
MLA Zhao, Peiji,et al."Amide N-glycosylation by Asm25, an N-glycosyltransferase of ansamitocins".CHEMISTRY & BIOLOGY 15.8(2008):863-874.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
201110260097.pdf(1375KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Peiji]的文章
[Bai, Linquan]的文章
[Ma, Juan]的文章
百度学术
百度学术中相似的文章
[Zhao, Peiji]的文章
[Bai, Linquan]的文章
[Ma, Juan]的文章
必应学术
必应学术中相似的文章
[Zhao, Peiji]的文章
[Bai, Linquan]的文章
[Ma, Juan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。