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Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids
Liu, Jin1,4; Gerken, Henri2; Huang, Junchao3; Chen, Feng4
Corresponding AuthorLiu, J (reprint author), Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA. ; jliu@umces.edu ; sfchencoe@pku.edu.cn
2013-05-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume48Issue:5-6Pages:788-795
AbstractA phytoene desaturase (PDS) gene was cloned and characterized from the unicellular green microalga Chlamydomonas reinhardtii. Functional complementation analysis revealed C reinhardtii PDS (CrPDS) catalyzes the conversion of phytoene to the colored carotenoid zeta-carotene. A single amino acid substitution, L505F, enhanced its desaturation activity by 29%, as indicated by an in vitro enzymatic assay. In addition, CrPDS-L505F exhibited 27.7-fold higher resistance to the herbicide norflurazon. Glass bead-mediated delivery displayed a high transformation efficiency of C reinhardtii with CrPDS-L505F, demonstrating clearly that the engineered endogenous CrPDS is a dominant selectable marker for C. reinhardtii and possibly for other green algae. Furthermore, the expression of PDS could enhance the intracellular carotenoid accumulation of transformants, opening up the possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae. (C) 2013 Elsevier Ltd. All rights reserved.
KeywordChlamydomonas Reinhardtii Carotenoids Norflurazon Phytoene Desaturase Selectable Marker Site-directed Mutation
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
DOI10.1016/j.procbio.2013.04.020
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000321423600006
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/16843
Collection资源植物与生物技术所级重点实验室
Affiliation1.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA
2.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ USA
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jin,Gerken, Henri,Huang, Junchao,et al. Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids[J]. PROCESS BIOCHEMISTRY,2013,48(5-6):788-795.
APA Liu, Jin,Gerken, Henri,Huang, Junchao,&Chen, Feng.(2013).Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids.PROCESS BIOCHEMISTRY,48(5-6),788-795.
MLA Liu, Jin,et al."Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids".PROCESS BIOCHEMISTRY 48.5-6(2013):788-795.
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