Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids
Liu, Jin1,4; Gerken, Henri2; Huang, Junchao3; Chen, Feng4
2013-05-01
发表期刊PROCESS BIOCHEMISTRY
ISSN1359-5113
卷号48期号:5-6页码:788-795
摘要A phytoene desaturase (PDS) gene was cloned and characterized from the unicellular green microalga Chlamydomonas reinhardtii. Functional complementation analysis revealed C reinhardtii PDS (CrPDS) catalyzes the conversion of phytoene to the colored carotenoid zeta-carotene. A single amino acid substitution, L505F, enhanced its desaturation activity by 29%, as indicated by an in vitro enzymatic assay. In addition, CrPDS-L505F exhibited 27.7-fold higher resistance to the herbicide norflurazon. Glass bead-mediated delivery displayed a high transformation efficiency of C reinhardtii with CrPDS-L505F, demonstrating clearly that the engineered endogenous CrPDS is a dominant selectable marker for C. reinhardtii and possibly for other green algae. Furthermore, the expression of PDS could enhance the intracellular carotenoid accumulation of transformants, opening up the possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae. (C) 2013 Elsevier Ltd. All rights reserved.
关键词Chlamydomonas Reinhardtii Carotenoids Norflurazon Phytoene Desaturase Selectable Marker Site-directed Mutation
资助信息985 Project of Peking University; State Oceanic Administration of China; 863 Project of the Ministry of Science and Technology of China
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
DOI10.1016/j.procbio.2013.04.020
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号WOS:000321423600006
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被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/16843
专题资源植物与生物技术所级重点实验室
作者单位1.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA
2.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ USA
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
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Liu, Jin,Gerken, Henri,Huang, Junchao,et al. Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids[J]. PROCESS BIOCHEMISTRY,2013,48(5-6):788-795.
APA Liu, Jin,Gerken, Henri,Huang, Junchao,&Chen, Feng.(2013).Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids.PROCESS BIOCHEMISTRY,48(5-6),788-795.
MLA Liu, Jin,et al."Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids".PROCESS BIOCHEMISTRY 48.5-6(2013):788-795.
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