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题名: Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids
作者: Liu, Jin1, 4; Gerken, Henri2; Huang, Junchao3; Chen, Feng4
刊名: PROCESS BIOCHEMISTRY
关键词: Chlamydomonas reinhardtii ; Carotenoids ; Norflurazon ; Phytoene desaturase ; Selectable marker ; Site-directed mutation
英文摘要: A phytoene desaturase (PDS) gene was cloned and characterized from the unicellular green microalga Chlamydomonas reinhardtii. Functional complementation analysis revealed C reinhardtii PDS (CrPDS) catalyzes the conversion of phytoene to the colored carotenoid zeta-carotene. A single amino acid substitution, L505F, enhanced its desaturation activity by 29%, as indicated by an in vitro enzymatic assay. In addition, CrPDS-L505F exhibited 27.7-fold higher resistance to the herbicide norflurazon. Glass bead-mediated delivery displayed a high transformation efficiency of C reinhardtii with CrPDS-L505F, demonstrating clearly that the engineered endogenous CrPDS is a dominant selectable marker for C. reinhardtii and possibly for other green algae. Furthermore, the expression of PDS could enhance the intracellular carotenoid accumulation of transformants, opening up the possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae. (C) 2013 Elsevier Ltd. All rights reserved.
出版日期: 2013-05-01
卷号: 48, 期号:5-6, 页码:788-795
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
ISSN号: 1359-5113
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/16843
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21201 USA
2.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ USA
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Jin; Gerken, Henri; Huang, Junchao; Chen, Feng.Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids,PROCESS BIOCHEMISTRY,2013,48(5-6):788-795
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