Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews
Li, Guoping1,2,3; Luo, Huairong4; Sun, Guotao3; Wu, Guisheng; Wu, Gang; Wang, Yan1,2; Man, Yong1,2; Wang, Shu1,2; Li, Jian1,2; Chen, Baosheng3
2013-09-01
发表期刊MOLECULAR BIOLOGY REPORTS
ISSN0301-4851
卷号40期号:9页码:5429-5438
摘要Apolipoprotein AV (apoAV) modulates plasma triglyceride levels, which is an independent risk factor for cardiovascular disease. ApoAV is also involved in atherosclerosis lesion formation. In order to systematically evaluate the apolipoprotein-related gene profile in tree shrew, a model for its insusceptibility to atherosclerosis, we performed apoAV cloning and characterization. The full-length cDNA of apoAV was identified using SMART-RACE. ApoAV cDNA sequence revealed two transcripts, 1,948 and 1,397 base pairs, due to alternative polyadenylation. These two transcripts share the same open reading frame (ORF), which encodes a 369-amino acid protein with high identity to human apoAV (75 %), including a 23-amino acid N-terminal signal peptide. ApoAV is expressed exclusively in the liver. Mature apoAV was expressed in E. coli BL21(DE3) and purified by Ni-chelated resin. Lipoprotein lipase activity was significantly stimulated by this recombinant protein. The full-length ORF of apoAV was cloned into pDsRed-monomer-N1 vector with a red fluorescent protein tag and was primarily localized in cytoplasm of hepG2 cells. The successful cloning, expression and localization of apoAV in tree shrew has laid down the foundation for further investigation on its structure and functions.
关键词Apolipoprotein Av Cdna Sequence Hdl-c Recombinant Protein Tree Shrew Triglyceride
资助信息National Basic Research Program of China [2012CB517502, 2006CB503801]; National Natural Science Foundation of China [81270948, 81070634, 81170381]; Beijing Hospital Youth Initial Funding [BJ-2011-30]
学科领域Biochemistry & Molecular Biology
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000323504000021
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/16827
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Beijing Hosp, Key Lab Geriatr, Beijing 100730, Peoples R China
2.Minist Hlth, Beijing Inst Geriatr, Beijing 100730, Peoples R China
3.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci,Natl Lab Med Mol Biol, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100005, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
5.Chinese Acad Sci & Yunnan Prov, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China
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Li, Guoping,Luo, Huairong,Sun, Guotao,et al. Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews[J]. MOLECULAR BIOLOGY REPORTS,2013,40(9):5429-5438.
APA Li, Guoping.,Luo, Huairong.,Sun, Guotao.,Wu, Guisheng.,Wu, Gang.,...&Chen, Baosheng.(2013).Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews.MOLECULAR BIOLOGY REPORTS,40(9),5429-5438.
MLA Li, Guoping,et al."Cloning and characterization of a novel apolipoprotein gene, apolipoprotein AV, in tree shrews".MOLECULAR BIOLOGY REPORTS 40.9(2013):5429-5438.
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