Metabolic engineering of tomato for high-yield production of astaxanthin

	Metabolic engineering of tomato for high-yield production of astaxanthin
	Huang, Jun-Chao1 ; Zhong, Yu-Juan 2,3; Liu, Jin 2; Sandmann, Gerhard 4; Chen, Feng 5
通讯作者	Chen, F (reprint author), Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China. ; sfchencoe@pku.edu.cn
	2013-05-01
发表期刊	METABOLIC ENGINEERING
ISSN	1096-7176
卷号	17 页码:59-67
摘要	Dietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nutritious tomato by modifying the intrinsic carotenes to astaxanthin, a high-value ketocarotenoid rarely found in plants. This was achieved by co-expression of the algal beta-carotene ketolase from Chlamydomonas reinhardtii and beta-carotene hydroxylase from Haematococcus pluvialis, a unique pair of enzymes identified to co-operate perfectly in converting p-carotene to astaxanthin by functional complementation in Escherichia coli. Expression of the two enzymes in tomato up-regulated most intrinsic carotenogenic genes, and efficiently directed carbon flux into carotenoids, leading to massive accumulations of mostly free astaxanthin in leaves (3.12 mg/g) but esterified astaxanthin in fruits (16.1 mg/g) and a 16-fold increase of total carotenoid capacity therein without affecting the plant normal growth and development. This study opened up the possibility of employing crop plants as green factories for economical production of astaxanthin. (C) 2013 Elsevier Inc. All rights reserved.
关键词	Astaxanthin Beta-carotene Ketolase Beta-carotene Hydroxylase Tomato
学科领域	Biotechnology & Applied Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000318583700007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16605
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China 2.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 3.Guangdong Acad Agr Sci, Inst Vegetable Res, Guangzhou, Guangdong, Peoples R China 4.Goethe Univ Frankfurt, Biosynth Grp, D-60054 Frankfurt, Germany 5.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,et al. Metabolic engineering of tomato for high-yield production of astaxanthin[J]. METABOLIC ENGINEERING,2013,17:59-67.
APA	Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,Sandmann, Gerhard,&Chen, Feng.(2013).Metabolic engineering of tomato for high-yield production of astaxanthin.METABOLIC ENGINEERING,17,59-67.
MLA	Huang, Jun-Chao,et al."Metabolic engineering of tomato for high-yield production of astaxanthin".METABOLIC ENGINEERING 17(2013):59-67.

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