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Metabolic engineering of tomato for high-yield production of astaxanthin
Huang, Jun-Chao1; Zhong, Yu-Juan2,3; Liu, Jin2; Sandmann, Gerhard4; Chen, Feng5
Corresponding AuthorChen, F (reprint author), Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China. ; sfchencoe@pku.edu.cn
2013-05-01
Source PublicationMETABOLIC ENGINEERING
ISSN1096-7176
Volume17Pages:59-67
AbstractDietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nutritious tomato by modifying the intrinsic carotenes to astaxanthin, a high-value ketocarotenoid rarely found in plants. This was achieved by co-expression of the algal beta-carotene ketolase from Chlamydomonas reinhardtii and beta-carotene hydroxylase from Haematococcus pluvialis, a unique pair of enzymes identified to co-operate perfectly in converting p-carotene to astaxanthin by functional complementation in Escherichia coli. Expression of the two enzymes in tomato up-regulated most intrinsic carotenogenic genes, and efficiently directed carbon flux into carotenoids, leading to massive accumulations of mostly free astaxanthin in leaves (3.12 mg/g) but esterified astaxanthin in fruits (16.1 mg/g) and a 16-fold increase of total carotenoid capacity therein without affecting the plant normal growth and development. This study opened up the possibility of employing crop plants as green factories for economical production of astaxanthin. (C) 2013 Elsevier Inc. All rights reserved.
KeywordAstaxanthin Beta-carotene Ketolase Beta-carotene Hydroxylase Tomato
Subject AreaBiotechnology & Applied Microbiology
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000318583700007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/16605
Collection资源植物与生物技术所级重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
2.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
3.Guangdong Acad Agr Sci, Inst Vegetable Res, Guangzhou, Guangdong, Peoples R China
4.Goethe Univ Frankfurt, Biosynth Grp, D-60054 Frankfurt, Germany
5.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,et al. Metabolic engineering of tomato for high-yield production of astaxanthin[J]. METABOLIC ENGINEERING,2013,17:59-67.
APA Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,Sandmann, Gerhard,&Chen, Feng.(2013).Metabolic engineering of tomato for high-yield production of astaxanthin.METABOLIC ENGINEERING,17,59-67.
MLA Huang, Jun-Chao,et al."Metabolic engineering of tomato for high-yield production of astaxanthin".METABOLIC ENGINEERING 17(2013):59-67.
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