| Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae | |
Li Xing-Yu1; Mao, Zi-Chao1; Wang, Yue-Hu2 ; Wu, Yi-Xing1; He, Yue-Qiu1; Long, Chun-Lin2,3
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| 通讯作者 | He, YQ (reprint author), Yunnan Agr Univ, Key Lab Agr Biodivers & Pests Control, Kunming 650201, Peoples R China. ; ynfb2007@163.com ; long@mail.kib.ac.cn |
| 2013-03-01 | |
| 发表期刊 | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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| ISSN | 1017-7825 |
| 卷号 | 23期号:3页码:313-321 |
| 摘要 | Bacillus subtilis XF-1, a strain with demonstrated ability to control clubroot disease caused by Plasmodiophora brassicae, was studied to elucidate its mechanism of antifungal activity against P brassicae. Fengycin-type cydopeptides (FTCPs), a well-known class of compounds with strong fungitoxic activity, were purified by acid precipitation, methanol extraction, and chromatographic separation. Eight homologs of fengycin, seven homologs of dehydroxyfengycin, and six unknown FTCPs were characterized with LC/ESI-MS, LC/ESI-MS/MS, and NMR. FTCPs (250 mu/ml) were used to treat the resting spores of P. brassicae (10(7)/ml) by detecting leakage of the cytoplasm components and cell destruction. After 12 h treatment, the absorbencies at 260 nm (A(260)) and at 280 nm (A(280)) increased gradually to approaching the maximum of absorbance, accompanying the collapse of P. brassicae resting spores, and nearly no complete cells were observed at 24 h treatment. The results suggested that the cells could be cleaved by the FTCPs of B. subtilis XF-1, and the diversity of FTCPs was mainly attributed to a mechanism of clubroot disease biocontrol. |
| 关键词 | Clubroot Disease Fungitoxic Activity Biocontrol Dehydroxyfengycin Chromatography Separation |
| 学科领域 | Biotechnology & Applied Microbiology ; Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000316919500005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/16485 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Yunnan Agr Univ, Key Lab Agr Biodivers & Pests Control, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China 3.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,et al. Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2013,23(3):313-321. |
| APA | Li Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,Wu, Yi-Xing,He, Yue-Qiu,&Long, Chun-Lin.(2013).Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,23(3),313-321. |
| MLA | Li Xing-Yu,et al."Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 23.3(2013):313-321. |
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