Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae
Li Xing-Yu1; Mao, Zi-Chao1; Wang, Yue-Hu2; Wu, Yi-Xing1; He, Yue-Qiu1; Long, Chun-Lin2,3
2013-03-01
发表期刊JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN1017-7825
卷号23期号:3页码:313-321
摘要Bacillus subtilis XF-1, a strain with demonstrated ability to control clubroot disease caused by Plasmodiophora brassicae, was studied to elucidate its mechanism of antifungal activity against P brassicae. Fengycin-type cydopeptides (FTCPs), a well-known class of compounds with strong fungitoxic activity, were purified by acid precipitation, methanol extraction, and chromatographic separation. Eight homologs of fengycin, seven homologs of dehydroxyfengycin, and six unknown FTCPs were characterized with LC/ESI-MS, LC/ESI-MS/MS, and NMR. FTCPs (250 mu/ml) were used to treat the resting spores of P. brassicae (10(7)/ml) by detecting leakage of the cytoplasm components and cell destruction. After 12 h treatment, the absorbencies at 260 nm (A(260)) and at 280 nm (A(280)) increased gradually to approaching the maximum of absorbance, accompanying the collapse of P. brassicae resting spores, and nearly no complete cells were observed at 24 h treatment. The results suggested that the cells could be cleaved by the FTCPs of B. subtilis XF-1, and the diversity of FTCPs was mainly attributed to a mechanism of clubroot disease biocontrol.
关键词Clubroot Disease Fungitoxic Activity Biocontrol Dehydroxyfengycin Chromatography Separation
资助信息Ministry of Agriculture of China [2010029030]; Natural Science Foundation of Yunnan Province [2008CC024]; National Natural Science Foundation of China [20972166, 31070288, 31161140345]; Ministry of Education of China through its 111 and 985 programs [B08044, MUC 985-6, MUC 98506-01000101]
学科领域Biotechnology & Applied Microbiology ; Microbiology
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
WOS类目Biotechnology & Applied Microbiology ; Microbiology
WOS记录号WOS:000316919500005
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/16485
专题资源植物与生物技术所级重点实验室
作者单位1.Yunnan Agr Univ, Key Lab Agr Biodivers & Pests Control, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
3.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
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Li Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,et al. Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2013,23(3):313-321.
APA Li Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,Wu, Yi-Xing,He, Yue-Qiu,&Long, Chun-Lin.(2013).Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,23(3),313-321.
MLA Li Xing-Yu,et al."Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 23.3(2013):313-321.
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