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Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae
Li Xing-Yu1; Mao, Zi-Chao1; Wang, Yue-Hu2; Wu, Yi-Xing1; He, Yue-Qiu1; Long, Chun-Lin2,3
Corresponding AuthorHe, YQ (reprint author), Yunnan Agr Univ, Key Lab Agr Biodivers & Pests Control, Kunming 650201, Peoples R China. ; ynfb2007@163.com ; long@mail.kib.ac.cn
2013-03-01
Source PublicationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN1017-7825
Volume23Issue:3Pages:313-321
AbstractBacillus subtilis XF-1, a strain with demonstrated ability to control clubroot disease caused by Plasmodiophora brassicae, was studied to elucidate its mechanism of antifungal activity against P brassicae. Fengycin-type cydopeptides (FTCPs), a well-known class of compounds with strong fungitoxic activity, were purified by acid precipitation, methanol extraction, and chromatographic separation. Eight homologs of fengycin, seven homologs of dehydroxyfengycin, and six unknown FTCPs were characterized with LC/ESI-MS, LC/ESI-MS/MS, and NMR. FTCPs (250 mu/ml) were used to treat the resting spores of P. brassicae (10(7)/ml) by detecting leakage of the cytoplasm components and cell destruction. After 12 h treatment, the absorbencies at 260 nm (A(260)) and at 280 nm (A(280)) increased gradually to approaching the maximum of absorbance, accompanying the collapse of P. brassicae resting spores, and nearly no complete cells were observed at 24 h treatment. The results suggested that the cells could be cleaved by the FTCPs of B. subtilis XF-1, and the diversity of FTCPs was mainly attributed to a mechanism of clubroot disease biocontrol.
KeywordClubroot Disease Fungitoxic Activity Biocontrol Dehydroxyfengycin Chromatography Separation
Subject AreaBiotechnology & Applied Microbiology ; Microbiology
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000316919500005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/16485
Collection资源植物与生物技术所级重点实验室
Affiliation1.Yunnan Agr Univ, Key Lab Agr Biodivers & Pests Control, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
3.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Li Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,et al. Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2013,23(3):313-321.
APA Li Xing-Yu,Mao, Zi-Chao,Wang, Yue-Hu,Wu, Yi-Xing,He, Yue-Qiu,&Long, Chun-Lin.(2013).Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,23(3),313-321.
MLA Li Xing-Yu,et al."Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 23.3(2013):313-321.
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