Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

	Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products
	Li Wen-gui 1; Dai Fei-yan 1; Cheng Yong-xian 2; Yin Ge-fen 1; Bi Jun-long 1; Li Dian-peng 3
通讯作者	Cheng, YX (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China. ; yxcheng@mail.kib.ac.cn
	2013-04-01
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	29 期号:2 页码:290-293
摘要	Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC50) values of 7.9 and 39.4 mu mol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.
关键词	Porcine Reproductive And Respiratory Syndrome Virus(Prrsv) Natural Product Cytopathic Effect Antiviral Activity
学科领域	Chemistry
收录类别	SCI
语种	英语
WOS记录号	WOS:000317165900021
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/16469
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Peoples R China 3.Guangxi Inst Bot, Guangxi Key Lab Funct Phytochem Res & Utilizat, Guilin 541006, Peoples R China
推荐引用方式 GB/T 7714	Li Wen-gui,Dai Fei-yan,Cheng Yong-xian,et al. Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2013,29(2):290-293.
APA	Li Wen-gui,Dai Fei-yan,Cheng Yong-xian,Yin Ge-fen,Bi Jun-long,&Li Dian-peng.(2013).Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,29(2),290-293.
MLA	Li Wen-gui,et al."Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 29.2(2013):290-293.

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