Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis
Liu, Jin1,4; Sun, Zheng2; Zhong, Yujuan2; Huang, Junchao3; Hu, Qiang4; Chen, Feng1
2012-12-01
发表期刊PLANTA
ISSN0032-0935
卷号236期号:6页码:1665-1676
摘要The green alga Chlorella zofingiensis can accumulate high level of oleic acid (OA, C18:1a-(39)) rich oils in response to stress conditions. To understand the regulation of biosynthesis of fatty acid in particular OA at the molecular level, we cloned and characterized the stearoyl acyl carrier protein (ACP) desaturase (SAD) responsible for OA formation through desaturation of stearic acid (C18:0) from C. zofingiensis. Southern blot indicated that the C. zofingiensis genome contained a single copy of SAD, from which the deduced amino acid sequence shared high identity to the corresponding homologs from other microalgae and higher plants. The desaturation activity of SAD was demonstrated in vitro using C18:0-ACP as a substrate. Stress conditions such as high light (HL), nitrogen deficiency (N-), or combination of HL and N- (HL + N-) drastically up-regulated the transcripts of biotin carboxylase (BC, a subunit of ACCase) and SAD, and therefore induced considerably the cellular accumulation of total fatty acids including OA. Glucose (50 mM) gave rise to the similar up-regulation of the two genes and induction of fatty acid accumulation. The accumulation of intracellular reactive oxygen species was found to be associated with the up-regulation of genes. This is the first report of characterization of Chlorella-derived SAD and the results may contribute to understanding of the mechanisms involved in fatty acid/lipid biosynthesis in microalgae.
关键词Chlorella Zofingiensis High Light Nitrogen Deficiency Oleic Acid Stearoyl-acyl Carrier Protein Desaturase
资助信息985 Project of Peking University; State Oceanic Administration of China
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15984
专题资源植物与生物技术所级重点实验室
作者单位1.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
2.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
4.Arizona State Univ Polytech Campus, Dept Appl Sci & Math, Mesa, AZ USA
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Liu, Jin,Sun, Zheng,Zhong, Yujuan,et al. Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis[J]. PLANTA,2012,236(6):1665-1676.
APA Liu, Jin,Sun, Zheng,Zhong, Yujuan,Huang, Junchao,Hu, Qiang,&Chen, Feng.(2012).Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis.PLANTA,236(6),1665-1676.
MLA Liu, Jin,et al."Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis".PLANTA 236.6(2012):1665-1676.
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