Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis
Zhang, Wei-Dong1; Wang, Qing1; Wang, Ying1; Wang, Xiao-Juan1; Pu, Jian-Xin2; Gu, Yi1; Wang, Rong1
2012-09-01
发表期刊JOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
卷号35期号:17页码:2203-2209
摘要Lignans in the drug Fructus Schisandrae chinensis (FSC) exhibit potent biological activities such as antihepatotoxic, antiasthmatic, and antigastric ulcer. An ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method has been developed to evaluate the quality of FSC through simultaneous qualitative and quantitative analysis of 15 lignans, including schizandrin A, B, and C; schizandrol A and B; gomisin B, C, D, E, G, H, J, and N; tigloylgomisin H; and angeloylgomisin H. The compounds were separated on a Zorbax Eclipse Plus C18 (2.1 x 100 mm, 1.8 mu m) column with a gradient elution of acetonitrile and 0.1% formic acid. Lignans were identified through their retention times, accurate mass data, and characteristic ions by comparison with a reference substance. All calibration curves showed perfect linear regression (r2 > 0.99) within the test range. The limits of detection and quantitation fell in the ranges of 0.14 ng/mL for all the analytes with an injection of 10 mu L. Good results were obtained with respect to repeatability (relative standard deviation <4.6%) and recovery (85.58-105.82%). Meanwhile, the entire sample analysis time was less than 10 min. This developed method provided a new basis for the overall assessment of the quality of FSC.
关键词Fructus Schisandrae Chinensis Lignans Qualitative And Quantitative Analysis Uhplc Q-tof Ms
资助信息National Science Foundation of China
DOI10.1002/jssc.201200393
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000308105500006
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15700
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Fourth Mil Med Univ, Dept Pharm, Sch Stomatol, Xian 710032, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Yunnan, Peoples R China
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Zhang, Wei-Dong,Wang, Qing,Wang, Ying,et al. Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(17):2203-2209.
APA Zhang, Wei-Dong.,Wang, Qing.,Wang, Ying.,Wang, Xiao-Juan.,Pu, Jian-Xin.,...&Wang, Rong.(2012).Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis.JOURNAL OF SEPARATION SCIENCE,35(17),2203-2209.
MLA Zhang, Wei-Dong,et al."Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis".JOURNAL OF SEPARATION SCIENCE 35.17(2012):2203-2209.
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