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题名: Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis
作者: Zhang, Wei-Dong1; Wang, Qing1; Wang, Ying1; Wang, Xiao-Juan1; Pu, Jian-Xin2; Gu, Yi1; Wang, Rong1
刊名: JOURNAL OF SEPARATION SCIENCE
关键词: Fructus Schisandrae chinensis ; Lignans ; Qualitative and quantitative analysis ; UHPLC ; Q-TOF MS
英文摘要: Lignans in the drug Fructus Schisandrae chinensis (FSC) exhibit potent biological activities such as antihepatotoxic, antiasthmatic, and antigastric ulcer. An ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method has been developed to evaluate the quality of FSC through simultaneous qualitative and quantitative analysis of 15 lignans, including schizandrin A, B, and C; schizandrol A and B; gomisin B, C, D, E, G, H, J, and N; tigloylgomisin H; and angeloylgomisin H. The compounds were separated on a Zorbax Eclipse Plus C18 (2.1 x 100 mm, 1.8 mu m) column with a gradient elution of acetonitrile and 0.1% formic acid. Lignans were identified through their retention times, accurate mass data, and characteristic ions by comparison with a reference substance. All calibration curves showed perfect linear regression (r2 > 0.99) within the test range. The limits of detection and quantitation fell in the ranges of 0.14 ng/mL for all the analytes with an injection of 10 mu L. Good results were obtained with respect to repeatability (relative standard deviation <4.6%) and recovery (85.58-105.82%). Meanwhile, the entire sample analysis time was less than 10 min. This developed method provided a new basis for the overall assessment of the quality of FSC.
出版日期: 2012-09-01
卷号: 35, 期号:17, 页码:2203-2209
DOI标识: 10.1002/jssc.201200393
语种: 英语
收录类别: SCI
ISSN号: 1615-9306
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/15700
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Fourth Mil Med Univ, Dept Pharm, Sch Stomatol, Xian 710032, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Yunnan, Peoples R China

Recommended Citation:
Zhang, Wei-Dong; Wang, Qing; Wang, Ying; Wang, Xiao-Juan; Pu, Jian-Xin; Gu, Yi; Wang, Rong.Application of ultrahigh-performance liquid chromatography coupled with mass spectrometry for analysis of lignans and quality control of Fructus Schisandrae chinensis,JOURNAL OF SEPARATION SCIENCE,2012,35(17):2203-2209
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