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题名: Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers
作者: Guo, Yu1, 2; Li, Yali1, 3; Huang, Yuan1; Jarvis, Devra4; Sato, Kazuhiro5; Kato, Kenji6; Tsuyuzaki, Hiroshi7; Chen, Lijuan2; Long, Chunlin1, 8
刊名: GENETIC RESOURCES AND CROP EVOLUTION
关键词: Genetic diversity ; Hordeum vulgare ; Hulless barley ; Shangri-la region
英文摘要: For adding the hulless barley resources of Shangri-la region to the global barley resource library, a basic work was done by us to assess their genetic diversity of this region. The genetic diversity of 60 hulless barley samples collected from three counties in Shangri-la region of Yunnan Province, were studied using SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism) markers. A total of 70 alleles were detected for 19 pairs of SSR primers, and 525 band containing 464 polymorphic bands were revealed for 5 pairs of AFLP primers. The value of polymorphism information content (PIC) ranged from 0.03 to 0.86 for SSR primers. The total numbers of alleles were 51, 55, 43 in three populations and the polymorphic bands were 188, 205 and 141. The genetic distances and genetic identity among the three populations showed their close relationship. The gene diversity among populations relative to the total population diversity (Gst) was 0.13 for SSR markers and 0.02 for AFLP markers and indicated that just 13 and 2% variations were among populations, respectively. The UPGMA cluster analysis revealed that all of the samples grouped randomly rather than clustered into distinct groups corresponding to their populations, row types and spring/fall types. We concluded that there was high genetic diversity in the population of Shangri-la region and the formation of diversity was related to complex environment and inhabitants' traditional practices.
出版日期: 2012-10-01
卷号: 59, 期号:7, 页码:1543-1552
DOI标识: 10.1007/s10722-011-9783-5
语种: 英语
ISSN号: 0925-9864
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/15658
Appears in Collections:资源植物与生物技术所级重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650204, Peoples R China
2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Biovers Int, I-00057 Rome, Italy
5.Okayama Univ, Bioresources Res Inst, Kurashiki, Okayama 127100046, Japan
6.Okayama Univ, Fac Agr, Okayama 7008530, Japan
7.Akita Prefectural Univ, Fac Bioresource Sci, Akita 0100444, Japan
8.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China

Recommended Citation:
Guo, Yu; Li, Yali; Huang, Yuan; Jarvis, Devra; Sato, Kazuhiro; Kato, Kenji; Tsuyuzaki, Hiroshi; Chen, Lijuan; Long, Chunlin.Genetic diversity analysis of hulless barley from Shangri-la region revealed by SSR and AFLP markers,GENETIC RESOURCES AND CROP EVOLUTION,2012,59(7):1543-1552
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