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题名: Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds
作者: Li, HM ; Zeng, ZH ; Hu, Z ; Wang, DC
刊名: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
关键词: RIBOSOME-INACTIVATING PROTEINS ; PHYTOLACCA-AMERICANA ; MACROMOLECULAR CRYSTALLOGRAPHY ; WEISSENBERG CAMERA ; GENOMIC CLONE ; PURIFICATION
英文摘要: Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine. Good quality crystals of PAP-S were grown at high protein concentration (100 mg ml(-1)) and high temperature (306 K). The crystals have space group I222 and cell parameters a = 78.7, b = 85.2 and c = 93.0 Angstrom. An X-ray diffraction data set with resolution up to 1.8 Angstrom was collected. This high-resolution data will help to locate the sugars bound to the protein and provide accurate structural data for understanding structure-function relationships of PAP-S.
出版日期: 1998
卷号: 54, 期号:1, 页码:137-139
语种: 英语
收录类别: SCI
学科分类: Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography
ISSN号: 0907-4449
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/15498
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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Li, HM; Zeng, ZH; Hu, Z; Wang, DC.Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds,ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,1998,54(1):137-139
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