Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds
Li, HM ; Zeng, ZH ; Hu, Z ; Wang, DC
1998
发表期刊ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN0907-4449
卷号54期号:1页码:137-139
摘要Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine. Good quality crystals of PAP-S were grown at high protein concentration (100 mg ml(-1)) and high temperature (306 K). The crystals have space group I222 and cell parameters a = 78.7, b = 85.2 and c = 93.0 Angstrom. An X-ray diffraction data set with resolution up to 1.8 Angstrom was collected. This high-resolution data will help to locate the sugars bound to the protein and provide accurate structural data for understanding structure-function relationships of PAP-S.
关键词Ribosome-inactivating Proteins Phytolacca-americana Macromolecular Crystallography Weissenberg Camera Genomic Clone Purification
学科领域Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15498
专题植物化学与西部植物资源持续利用国家重点实验室
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GB/T 7714
Li, HM,Zeng, ZH,Hu, Z,et al. Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,1998,54(1):137-139.
APA Li, HM,Zeng, ZH,Hu, Z,&Wang, DC.(1998).Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,54(1),137-139.
MLA Li, HM,et al."Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 54.1(1998):137-139.
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