| Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds |
| Li, HM
; Zeng, ZH
; Hu, Z
; Wang, DC
|
| 通讯作者 | Li, HM (reprint author), Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China.
|
| 1998
|
| 发表期刊 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
 |
| ISSN | 0907-4449
|
| 卷号 | 54期号:1页码:137-139 |
| 摘要 | Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine. Good quality crystals of PAP-S were grown at high protein concentration (100 mg ml(-1)) and high temperature (306 K). The crystals have space group I222 and cell parameters a = 78.7, b = 85.2 and c = 93.0 Angstrom. An X-ray diffraction data set with resolution up to 1.8 Angstrom was collected. This high-resolution data will help to locate the sugars bound to the protein and provide accurate structural data for understanding structure-function relationships of PAP-S. |
| 关键词 | Ribosome-inactivating Proteins
Phytolacca-americana
Macromolecular Crystallography
Weissenberg Camera
Genomic Clone
Purification
|
| 学科领域 | Biochemical Research Methods
; Biochemistry & Molecular Biology
; Biophysics
; Crystallography
|
| 收录类别 | SCI
|
| 语种 | 英语
|
| 文献类型 | 期刊论文
|
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/15498
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| 专题 | 植物化学与西部植物资源持续利用国家重点实验室
|
推荐引用方式
GB/T 7714 |
Li, HM,Zeng, ZH,Hu, Z,et al. Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,1998,54(1):137-139.
|
| APA |
Li, HM,Zeng, ZH,Hu, Z,&Wang, DC.(1998).Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,54(1),137-139.
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| MLA |
Li, HM,et al."Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 54.1(1998):137-139.
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