Inhibition of enterovirus 71 replication by chrysosplenetin and penduletin
Zhu, Qin-Chang1,3; Wang, Yi1,4; Liu, Ya-Ping2; Zhang, Rui-Qi1; Li, Xiang1; Su, Wen-Han1; Long, Fei1; Luo, Xiao-Dong2; Peng, Tao1,4
2011-10-09
发表期刊EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN0928-0987
卷号44期号:3页码:392-398
摘要In recent years, enterovirus 71 (EV71) infections have caused an increasing epidemic in young children, accompanying with more severe nervous system disease and more deaths. Unfortunately, there is no specific medication for it so far. Here we investigated the anti-EV71 activity of chrysosplenetin and penduletin, two o-methylated flavonols isolated from the leaves of Laggera pterodonta. These two compounds were found to have strong activity in vitro against EV71 with low cytotoxicity. In the cytopathic effect (CPE) inhibition assays, both plaque reduction assay and virus yield inhibition assay, the compounds showed a similar 50% inhibitory concentration (IC(50)) value of about 0.20 mu M. The selectivity indices (SI) of chrysosplenetin and penduletin were 107.5 and 655.6 in African green monkey kidney (Vero) cells, and 69.5 and 200.5 in human rhabdomyosarcoma (RD) cells, accordingly. The preliminary mechanism analysis indicates that they function not through blocking virus entry or inactivating virus directly but inhibiting viral RNA replication. In the time-of-addition assay, both compounds inhibited progeny virus production and RNA replication by nearly 100% when introduced within 4 h post infection. In addition to EV71, both compounds inhibited several other human enteroviruses with similar efficacy. These findings provide a significant lead for the discovery of anti-EV71 drug. (C) 2011 Elsevier B.V. All rights reserved.
关键词Enterovirus Laggera Pterodonta Flavonol Antiviral Activity
资助信息National Basic Research Program (973)[2009CB522300, 2010C13530100]; Department of Education of Guangdong Province[GXZD0901]; Science and Technology Program of Guangzhou, China[2007Z1-E0111]
学科领域Pharmacology & Pharmacy
DOI10.1016/j.ejps.2011.08.030
收录类别SCI
语种英语
WOS研究方向Pharmacology & Pharmacy
WOS类目Pharmacology & Pharmacy
WOS记录号WOS:000296930000025
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15370
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Lab Viral Immunol, State Key Lab Resp Dis, Guangzhou 510530, Guangdong, Peoples R China
2.State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
3.Nagasaki Univ, Sch Pharmaceut Sci, Lab Chem Biofunct Mol, Nagasaki 8528521, Japan
4.S China United Vaccine Inst, Guangzhou 510633, Guangdong, Peoples R China
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Zhu, Qin-Chang,Wang, Yi,Liu, Ya-Ping,et al. Inhibition of enterovirus 71 replication by chrysosplenetin and penduletin[J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,2011,44(3):392-398.
APA Zhu, Qin-Chang.,Wang, Yi.,Liu, Ya-Ping.,Zhang, Rui-Qi.,Li, Xiang.,...&Peng, Tao.(2011).Inhibition of enterovirus 71 replication by chrysosplenetin and penduletin.EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,44(3),392-398.
MLA Zhu, Qin-Chang,et al."Inhibition of enterovirus 71 replication by chrysosplenetin and penduletin".EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES 44.3(2011):392-398.
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