| Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants | |
Huang, Junchao4 ; Zhong, Yujuan3; Sandmann, Gerhard2; Liu, Jin3; Chen, Feng1
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| 通讯作者 | Chen, F (reprint author), Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China. ; huangjc@mail.kib.ac.cn ; sfchencoe@pku.edu.cn |
| 2012-08-01 | |
| 发表期刊 | PLANTA
 |
| ISSN | 0032-0935 |
| 卷号 | 236期号:2页码:691-699 |
| 摘要 | beta-Carotene ketolase (BKT) catalyzes the rate-limiting steps for the biosynthesis of astaxanthin. Several bkt genes have been isolated and explored to modify plant carotenoids to astaxanthin with limited success. In this study, five algal BKT cDNAs were isolated and characterized for the engineering of high-yield astaxanthin in plants. The products of the cDNAs showed high similarity in sequence and enzymatic activity of converting beta-carotene into canthaxanthin. However, the enzymes exhibited extremely different activities in converting zeaxanthin into astaxanthin. Chlamydomonas reinhardtii BKT showed the highest conversion rate (ca 85 %), whereas, Neochloris wimmeri BKT exhibited very poor activity of ketolating zeaxanthin. Expression of C. reinhardtii BKT in tobacco led to a twofold increase of total carotenoids in the leaves with astaxanthin being the predominant. The bkt genes described here provide a valuable resource for metabolic engineering of plants as cell factories for astaxanthin production. |
| 关键词 | Astaxanthin Beta-carotene Ketolase Green Algae Plant |
| 学科领域 | Plant Sciences |
| DOI | 10.1007/s00425-012-1654-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306794100027 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/15123 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China 2.Goethe Univ Frankfurt, Biosynth Grp, Frankfurt, Germany 3.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Junchao,Zhong, Yujuan,Sandmann, Gerhard,et al. Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants[J]. PLANTA,2012,236(2):691-699. |
| APA | Huang, Junchao,Zhong, Yujuan,Sandmann, Gerhard,Liu, Jin,&Chen, Feng.(2012).Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants.PLANTA,236(2),691-699. |
| MLA | Huang, Junchao,et al."Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants".PLANTA 236.2(2012):691-699. |
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