Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants
Huang, Junchao4; Zhong, Yujuan3; Sandmann, Gerhard2; Liu, Jin3; Chen, Feng1
2012-08-01
发表期刊PLANTA
ISSN0032-0935
卷号236期号:2页码:691-699
摘要beta-Carotene ketolase (BKT) catalyzes the rate-limiting steps for the biosynthesis of astaxanthin. Several bkt genes have been isolated and explored to modify plant carotenoids to astaxanthin with limited success. In this study, five algal BKT cDNAs were isolated and characterized for the engineering of high-yield astaxanthin in plants. The products of the cDNAs showed high similarity in sequence and enzymatic activity of converting beta-carotene into canthaxanthin. However, the enzymes exhibited extremely different activities in converting zeaxanthin into astaxanthin. Chlamydomonas reinhardtii BKT showed the highest conversion rate (ca 85 %), whereas, Neochloris wimmeri BKT exhibited very poor activity of ketolating zeaxanthin. Expression of C. reinhardtii BKT in tobacco led to a twofold increase of total carotenoids in the leaves with astaxanthin being the predominant. The bkt genes described here provide a valuable resource for metabolic engineering of plants as cell factories for astaxanthin production.
关键词Astaxanthin Beta-carotene Ketolase Green Algae Plant
资助信息Kunming Institute of Botany of Chinese Academy of Sciences; "985" project of Peking University
学科领域Plant Sciences
DOI10.1007/s00425-012-1654-6
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000306794100027
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/15123
专题资源植物与生物技术所级重点实验室
作者单位1.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
2.Goethe Univ Frankfurt, Biosynth Grp, Frankfurt, Germany
3.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China
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Huang, Junchao,Zhong, Yujuan,Sandmann, Gerhard,et al. Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants[J]. PLANTA,2012,236(2):691-699.
APA Huang, Junchao,Zhong, Yujuan,Sandmann, Gerhard,Liu, Jin,&Chen, Feng.(2012).Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants.PLANTA,236(2),691-699.
MLA Huang, Junchao,et al."Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants".PLANTA 236.2(2012):691-699.
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