Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427
Shin, Jin Chul1,2; Na, Zhu1,3,4; Lee, Dongho5; Kim, Woncheol1,2; Lee, Kyeong1; Shen, Yue-Mao3; Paik, Sang-Gi2; Hong, Young-Soo1; Lee, Jung Joon1
2008-06-01
发表期刊JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN1017-7825
卷号18期号:6页码:1101-1108
摘要Geldanamycin and its analogs are important anticancer agents that inhibit the newly targeted heat-shock protein (Hsp) 90, which is a chaperone protein in eukaryotic cells. To resolve which geldanamycin biosynthetic genes are responsible for particular post-polyketide synthase (PKS) processing steps and in which order the reactions occur, we individually inactivated candidate genes in Streptomyces hygroscopicus subsp. duamyceticus JCM4427 and isolated and elucidated the structures of intermediates from each mutant. The results indicated that gel7 governs at least one of the benzoquinone ring oxidation steps. The gel16 was found to be involved in double-bond formation between C-4 and C-5 of 4,5-dihydrogeldanamycin, which confirmed our previous findings that this double bond is reduced during the post-PKS modification of the polyketide assembly. In addition, pro-geldanamycin, which does not possess a double bond at C-4/5, was purified from the gel7 and gel8 double-gene-inactivated mutant.
关键词Geldanamycin Biosynthesis Streptomyces Polyketide
学科领域Biotechnology & Applied Microbiology ; Microbiology
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
WOS类目Biotechnology & Applied Microbiology ; Microbiology
WOS记录号WOS:000257212000016
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/1491
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.KRIBB, Taejon 305806, South Korea
2.Chungnam Natl Univ, Taejon 305764, South Korea
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136705, South Korea
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Shin, Jin Chul,Na, Zhu,Lee, Dongho,et al. Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2008,18(6):1101-1108.
APA Shin, Jin Chul.,Na, Zhu.,Lee, Dongho.,Kim, Woncheol.,Lee, Kyeong.,...&Lee, Jung Joon.(2008).Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,18(6),1101-1108.
MLA Shin, Jin Chul,et al."Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 18.6(2008):1101-1108.
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