Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427
Shin, Jin Chul1,2; Na, Zhu1,3,4; Lee, Dongho5; Kim, Woncheol1,2; Lee, Kyeong1; Shen, Yue-Mao3; Paik, Sang-Gi2; Hong, Young-Soo1; Lee, Jung Joon1
2008-06-01
Source PublicationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN1017-7825
Volume18Issue:6Pages:1101-1108
AbstractGeldanamycin and its analogs are important anticancer agents that inhibit the newly targeted heat-shock protein (Hsp) 90, which is a chaperone protein in eukaryotic cells. To resolve which geldanamycin biosynthetic genes are responsible for particular post-polyketide synthase (PKS) processing steps and in which order the reactions occur, we individually inactivated candidate genes in Streptomyces hygroscopicus subsp. duamyceticus JCM4427 and isolated and elucidated the structures of intermediates from each mutant. The results indicated that gel7 governs at least one of the benzoquinone ring oxidation steps. The gel16 was found to be involved in double-bond formation between C-4 and C-5 of 4,5-dihydrogeldanamycin, which confirmed our previous findings that this double bond is reduced during the post-PKS modification of the polyketide assembly. In addition, pro-geldanamycin, which does not possess a double bond at C-4/5, was purified from the gel7 and gel8 double-gene-inactivated mutant.
KeywordGeldanamycin Biosynthesis Streptomyces Polyketide
Subject AreaBiotechnology & Applied Microbiology ; Microbiology
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000257212000016
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/1491
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.KRIBB, Taejon 305806, South Korea
2.Chungnam Natl Univ, Taejon 305764, South Korea
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136705, South Korea
Recommended Citation
GB/T 7714
Shin, Jin Chul,Na, Zhu,Lee, Dongho,et al. Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2008,18(6):1101-1108.
APA Shin, Jin Chul.,Na, Zhu.,Lee, Dongho.,Kim, Woncheol.,Lee, Kyeong.,...&Lee, Jung Joon.(2008).Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,18(6),1101-1108.
MLA Shin, Jin Chul,et al."Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 18.6(2008):1101-1108.
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