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题名: Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427
作者: Shin, Jin Chul1, 2; Na, Zhu1, 3, 4; Lee, Dongho5; Kim, Woncheol1, 2; Lee, Kyeong1; Shen, Yue-Mao3; Paik, Sang-Gi2; Hong, Young-Soo1; Lee, Jung Joon1
刊名: JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
关键词: Geldanamycin ; biosynthesis ; Streptomyces ; polyketide
英文摘要: Geldanamycin and its analogs are important anticancer agents that inhibit the newly targeted heat-shock protein (Hsp) 90, which is a chaperone protein in eukaryotic cells. To resolve which geldanamycin biosynthetic genes are responsible for particular post-polyketide synthase (PKS) processing steps and in which order the reactions occur, we individually inactivated candidate genes in Streptomyces hygroscopicus subsp. duamyceticus JCM4427 and isolated and elucidated the structures of intermediates from each mutant. The results indicated that gel7 governs at least one of the benzoquinone ring oxidation steps. The gel16 was found to be involved in double-bond formation between C-4 and C-5 of 4,5-dihydrogeldanamycin, which confirmed our previous findings that this double bond is reduced during the post-PKS modification of the polyketide assembly. In addition, pro-geldanamycin, which does not possess a double bond at C-4/5, was purified from the gel7 and gel8 double-gene-inactivated mutant.
出版日期: 2008-06-01
卷号: 18, 期号:6, 页码:1101-1108
语种: 英语
收录类别: SCI
学科分类: Biotechnology & Applied Microbiology; Microbiology
ISSN号: 1017-7825
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/1491
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.KRIBB, Taejon 305806, South Korea
2.Chungnam Natl Univ, Taejon 305764, South Korea
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136705, South Korea

Recommended Citation:
Shin, JC; Na, Z; Lee, D; Kim, W; Lee, K; Shen, YM; Paik, SG; Hong, YS; Lee, JJ.Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427,JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2008,18(6):1101-1108
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