Protoplast culture and paclitaxel production by Taxus yunnanensis

	Protoplast culture and paclitaxel production by Taxus yunnanensis
	Luo, JP ; Mu, Q ; Gu, YH
	1999
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
卷号	59 期号:1 页码:25-29
摘要	Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0 mg l(-1) 2,4-dichlorophenoxyacetic acid and 0.1 mg l(-1) kinetin. The planting density was 2.5-3.0x10(5) protoplasts per ml of culture medium. Cell-free extract from callus enhanced protoplast division and the highest plating efficiency was about 7%. Protoplast-derived colonies showed significant variations in both growth and paclitaxel content. A negative correlation existed between paclitaxel accumulation in colonies and their growth to some extent (r = -0.4485). Among 70 colonies isolated from the heterogeneous protoplast cultures, colony TY-7 accumulated the highest paclitaxel content. Paclitaxel accumulation in colony TY-7 was not great enough to produce paclitaxel for commercial purposes, however, success in inducing colony formation from T. yunnanensis protoplasts provides an opportunity to obtain cell lines with high paclitaxel productivity from mutagenized protoplast cultures.
关键词	Taxus Yunnanensis Protoplast-derived Colonies Paclitaxel
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000087181800004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/14591
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Dept Phytochem, Kunming 650204, Peoples R China
推荐引用方式 GB/T 7714	Luo, JP,Mu, Q,Gu, YH. Protoplast culture and paclitaxel production by Taxus yunnanensis[J]. PLANT CELL TISSUE AND ORGAN CULTURE,1999,59(1):25-29.
APA	Luo, JP,Mu, Q,&Gu, YH.(1999).Protoplast culture and paclitaxel production by Taxus yunnanensis.PLANT CELL TISSUE AND ORGAN CULTURE,59(1),25-29.
MLA	Luo, JP,et al."Protoplast culture and paclitaxel production by Taxus yunnanensis".PLANT CELL TISSUE AND ORGAN CULTURE 59.1(1999):25-29.

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