Protoplast culture and paclitaxel production by Taxus yunnanensis
Luo, JP; Mu, Q; Gu, YH
1999
发表期刊PLANT CELL TISSUE AND ORGAN CULTURE
卷号59期号:1页码:25-29
摘要Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0 mg l(-1) 2,4-dichlorophenoxyacetic acid and 0.1 mg l(-1) kinetin. The planting density was 2.5-3.0x10(5) protoplasts per ml of culture medium. Cell-free extract from callus enhanced protoplast division and the highest plating efficiency was about 7%. Protoplast-derived colonies showed significant variations in both growth and paclitaxel content. A negative correlation existed between paclitaxel accumulation in colonies and their growth to some extent (r = -0.4485). Among 70 colonies isolated from the heterogeneous protoplast cultures, colony TY-7 accumulated the highest paclitaxel content. Paclitaxel accumulation in colony TY-7 was not great enough to produce paclitaxel for commercial purposes, however, success in inducing colony formation from T. yunnanensis protoplasts provides an opportunity to obtain cell lines with high paclitaxel productivity from mutagenized protoplast cultures.
关键词Taxus Yunnanensis Protoplast-derived Colonies Paclitaxel
学科领域Biotechnology & Applied Microbiology ; Plant Sciences
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号WOS:000087181800004
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14591
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Dept Phytochem, Kunming 650204, Peoples R China
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GB/T 7714
Luo, JP,Mu, Q,Gu, YH. Protoplast culture and paclitaxel production by Taxus yunnanensis[J]. PLANT CELL TISSUE AND ORGAN CULTURE,1999,59(1):25-29.
APA Luo, JP,Mu, Q,&Gu, YH.(1999).Protoplast culture and paclitaxel production by Taxus yunnanensis.PLANT CELL TISSUE AND ORGAN CULTURE,59(1),25-29.
MLA Luo, JP,et al."Protoplast culture and paclitaxel production by Taxus yunnanensis".PLANT CELL TISSUE AND ORGAN CULTURE 59.1(1999):25-29.
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