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题名: Protoplast culture and paclitaxel production by Taxus yunnanensis
作者: Luo, JP; Mu, Q; Gu, YH
刊名: PLANT CELL TISSUE AND ORGAN CULTURE
关键词: Taxus yunnanensis ; protoplast-derived colonies ; paclitaxel
英文摘要: Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0 mg l(-1) 2,4-dichlorophenoxyacetic acid and 0.1 mg l(-1) kinetin. The planting density was 2.5-3.0x10(5) protoplasts per ml of culture medium. Cell-free extract from callus enhanced protoplast division and the highest plating efficiency was about 7%. Protoplast-derived colonies showed significant variations in both growth and paclitaxel content. A negative correlation existed between paclitaxel accumulation in colonies and their growth to some extent (r = -0.4485). Among 70 colonies isolated from the heterogeneous protoplast cultures, colony TY-7 accumulated the highest paclitaxel content. Paclitaxel accumulation in colony TY-7 was not great enough to produce paclitaxel for commercial purposes, however, success in inducing colony formation from T. yunnanensis protoplasts provides an opportunity to obtain cell lines with high paclitaxel productivity from mutagenized protoplast cultures.
出版日期: 1999
卷号: 59, 期号:1, 页码:25-29
语种: 英语
收录类别: SCI
学科分类: Biotechnology & Applied Microbiology; Plant Sciences
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/14591
Appears in Collections:植物化学与西部植物资源持续利用国家重点实验室_期刊论文

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作者单位: 1.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Dept Phytochem, Kunming 650204, Peoples R China

Recommended Citation:
Luo, JP; Mu, Q; Gu, YH.Protoplast culture and paclitaxel production by Taxus yunnanensis,PLANT CELL TISSUE AND ORGAN CULTURE,1999,59(1):25-29
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