| Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY | |
| Guo, Ava J. Y.; Choi, Roy C. Y.; Cheung, Anna W. H.; Chen, Vicky P.; Xu, Sherry L.; Dong, Tina T. X.; Chen, Ji J.3; Tsim, Karl W. K.1,2 | |
| Corresponding Author | Tsim, KWK (reprint author), Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay Rd, Hong Kong, Hong Kong, Peoples R China |
| 2011-08-12 | |
| Source Publication | JOURNAL OF BIOLOGICAL CHEMISTRY
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| ISSN | 0021-9258 |
| Volume | 286Issue:32Pages:27882-27893 |
| Abstract | Flavonoids, a group of natural compounds found in a variety of vegetables and herbal medicines, have been intensively reported on regarding their estrogen-like activities and particularly their ability to affect bone metabolism. Here, different subclasses of flavonoids were screened for their osteogenic properties by measuring alkaline phosphatase activity in cultured rat osteoblasts. The flavone baicalin derived mainly from the roots of Scutellaria baicalensis showed the strongest induction of alkaline phosphatase activity. In cultured osteoblasts, application of baicalin increased significantly the osteoblastic mineralization and the levels of mRNAs encoding the bone differentiation markers, including osteonectin, osteocalcin, and collagen type 1 alpha 1. Interestingly, the osteogenic effect of baicalin was not mediated by its estrogenic activity. In contrast, baicalin promoted osteoblastic differentiation via the activation of the Wnt/beta-catenin signaling pathway; the activation resulted in the phosphorylation of glycogen synthase kinase 3 beta and, subsequently, induced the nuclear accumulation of the beta-catenin, leading to the transcription activation of Wnt-targeted genes for osteogenesis. The baicalin-induced osteogenic effects were fully abolished by DKK-1, a blocker of Wnt/beta-catenin receptor. Moreover, baicalin also enhanced the mRNA expression of osteoprotegerin, which could regulate indirectly the activation of osteoclasts. Taken together, our results suggested that baicalin could act via Wnt/beta-catenin signaling to promote osteoblastic differentiation. The osteogenic flavonoids could be very useful in finding potential drugs, or food supplements, for treating post-menopausal osteoporosis. |
| Keyword | Hormone-replacement Therapy Bone Loss Postmenopausal Osteoporosis Scutellaria-baicalensis Heme Oxygenase-1 Gene-expression Protein Osteoprotegerin Genistein Cells |
| Subject Area | Biochemistry & Molecular Biology |
| DOI | 10.1074/jbc.M111.236281 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000293557800007 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/14493 |
| Collection | 植物化学与西部植物资源持续利用国家重点实验室 |
| Affiliation | 1.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Hong Kong, Peoples R China 3.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Guo, Ava J. Y.,Choi, Roy C. Y.,Cheung, Anna W. H.,et al. Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(32):27882-27893. |
| APA | Guo, Ava J. Y..,Choi, Roy C. Y..,Cheung, Anna W. H..,Chen, Vicky P..,Xu, Sherry L..,...&Tsim, Karl W. K..(2011).Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY.JOURNAL OF BIOLOGICAL CHEMISTRY,286(32),27882-27893. |
| MLA | Guo, Ava J. Y.,et al."Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY".JOURNAL OF BIOLOGICAL CHEMISTRY 286.32(2011):27882-27893. |
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