Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY
Guo, Ava J. Y.; Choi, Roy C. Y.; Cheung, Anna W. H.; Chen, Vicky P.; Xu, Sherry L.; Dong, Tina T. X.; Chen, Ji J.3; Tsim, Karl W. K.1,2
2011-08-12
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
卷号286期号:32页码:27882-27893
摘要Flavonoids, a group of natural compounds found in a variety of vegetables and herbal medicines, have been intensively reported on regarding their estrogen-like activities and particularly their ability to affect bone metabolism. Here, different subclasses of flavonoids were screened for their osteogenic properties by measuring alkaline phosphatase activity in cultured rat osteoblasts. The flavone baicalin derived mainly from the roots of Scutellaria baicalensis showed the strongest induction of alkaline phosphatase activity. In cultured osteoblasts, application of baicalin increased significantly the osteoblastic mineralization and the levels of mRNAs encoding the bone differentiation markers, including osteonectin, osteocalcin, and collagen type 1 alpha 1. Interestingly, the osteogenic effect of baicalin was not mediated by its estrogenic activity. In contrast, baicalin promoted osteoblastic differentiation via the activation of the Wnt/beta-catenin signaling pathway; the activation resulted in the phosphorylation of glycogen synthase kinase 3 beta and, subsequently, induced the nuclear accumulation of the beta-catenin, leading to the transcription activation of Wnt-targeted genes for osteogenesis. The baicalin-induced osteogenic effects were fully abolished by DKK-1, a blocker of Wnt/beta-catenin receptor. Moreover, baicalin also enhanced the mRNA expression of osteoprotegerin, which could regulate indirectly the activation of osteoclasts. Taken together, our results suggested that baicalin could act via Wnt/beta-catenin signaling to promote osteoblastic differentiation. The osteogenic flavonoids could be very useful in finding potential drugs, or food supplements, for treating post-menopausal osteoporosis.
关键词Hormone-replacement Therapy Bone Loss Postmenopausal Osteoporosis Scutellaria-baicalensis Heme Oxygenase-1 Gene-expression Protein Osteoprotegerin Genistein Cells
资助信息Research Grants Council of Hong Kong HKUST[6419/06M, 662608, N-HKUST629/07]; Croucher Foundation[CAS-CF07/08.SC03]
学科领域Biochemistry & Molecular Biology
DOI10.1074/jbc.M111.236281
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000293557800007
引用统计
被引频次:63[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14493
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
2.Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Hong Kong, Peoples R China
3.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
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Guo, Ava J. Y.,Choi, Roy C. Y.,Cheung, Anna W. H.,et al. Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(32):27882-27893.
APA Guo, Ava J. Y..,Choi, Roy C. Y..,Cheung, Anna W. H..,Chen, Vicky P..,Xu, Sherry L..,...&Tsim, Karl W. K..(2011).Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY.JOURNAL OF BIOLOGICAL CHEMISTRY,286(32),27882-27893.
MLA Guo, Ava J. Y.,et al."Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts AN ACTION VIA THE Wnt/beta-CATENIN SIGNALING PATHWAY".JOURNAL OF BIOLOGICAL CHEMISTRY 286.32(2011):27882-27893.
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