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Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH)
Wang, L; Li, YF; Tang, RH; Gu, ZJ
Corresponding Authoryzxj@vip.163.com
2004-09-01
Source PublicationACTA PHYTOTAXONOMICA SINICA
ISSN0529-1526
Volume42Issue:5Pages:419-426
Abstract18-26S rDNA loci were mapped on chromosomes in four species of Paris, and the number and position of rDNA sites in these species were compared for analysis of the distribution of the sites. All the plants were diploids, and the genome consisted of five chromosomes, A, B, C, D and E. (1) P. polyphylla var. yunnanensis, 2n = 10 = 6m + 4t. Two 18-26S rDNA loci were detected on the short arms of C and D chromosomes; (2) P. forrestii, 2n = 10 = 6m + 4t. One locus was detected on the long arm of B chromosome, and also two loci on the short arms of C and D chromosomes; (3) P. axialis. 2n = 10 = 6m(2sat) + 4t(2sat) + 1 - 2B. Two loci were detected on the short arms of C and D chromosomes. One locus was detected in the cell with two B-chromosomes (B), but none was detected in that with only one B chromosome, indicating that rRNA gene existed on B chromsome, and an unequal division occurred during mitotic cycle of B-chromosomes. (4) P. daliensis, 2n = 10 = 4m + 2sm + 2st + 2t. One locus was detected on the short arm of D chromosome. The signals of 18-26S rDNA appeared not only in the second constriction but also in the other regions of chromosome. It is noteworthy that one locus was detected in the terminal region on the short arm of C chromosome in all the four species studied.
KeywordParis Fluorescence In Situ Hybridization (Fish) 18-26s Rdna b Chromosome
Subject AreaPlant Sciences
Indexed BySCI
Language英语
WOS IDWOS:000224380800002
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/13501
Collection离退休
Affiliation1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
Recommended Citation
GB/T 7714
Wang, L,Li, YF,Tang, RH,et al. Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH)[J]. ACTA PHYTOTAXONOMICA SINICA,2004,42(5):419-426.
APA Wang, L,Li, YF,Tang, RH,&Gu, ZJ.(2004).Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH).ACTA PHYTOTAXONOMICA SINICA,42(5),419-426.
MLA Wang, L,et al."Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH)".ACTA PHYTOTAXONOMICA SINICA 42.5(2004):419-426.
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