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Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH)
Wang, L; Li, YF; Tang, RH; Gu, ZJ
2004-09-01
发表期刊ACTA PHYTOTAXONOMICA SINICA
ISSN0529-1526
卷号42期号:5页码:419-426
摘要18-26S rDNA loci were mapped on chromosomes in four species of Paris, and the number and position of rDNA sites in these species were compared for analysis of the distribution of the sites. All the plants were diploids, and the genome consisted of five chromosomes, A, B, C, D and E. (1) P. polyphylla var. yunnanensis, 2n = 10 = 6m + 4t. Two 18-26S rDNA loci were detected on the short arms of C and D chromosomes; (2) P. forrestii, 2n = 10 = 6m + 4t. One locus was detected on the long arm of B chromosome, and also two loci on the short arms of C and D chromosomes; (3) P. axialis. 2n = 10 = 6m(2sat) + 4t(2sat) + 1 - 2B. Two loci were detected on the short arms of C and D chromosomes. One locus was detected in the cell with two B-chromosomes (B), but none was detected in that with only one B chromosome, indicating that rRNA gene existed on B chromsome, and an unequal division occurred during mitotic cycle of B-chromosomes. (4) P. daliensis, 2n = 10 = 4m + 2sm + 2st + 2t. One locus was detected on the short arm of D chromosome. The signals of 18-26S rDNA appeared not only in the second constriction but also in the other regions of chromosome. It is noteworthy that one locus was detected in the terminal region on the short arm of C chromosome in all the four species studied.
关键词Paris Fluorescence In Situ Hybridization (Fish) 18-26s Rdna b Chromosome
学科领域Plant Sciences
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000224380800002
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13501
专题离退休
作者单位1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
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GB/T 7714
Wang, L,Li, YF,Tang, RH,et al. Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH)[J]. ACTA PHYTOTAXONOMICA SINICA,2004,42(5):419-426.
APA Wang, L,Li, YF,Tang, RH,&Gu, ZJ.(2004).Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH).ACTA PHYTOTAXONOMICA SINICA,42(5),419-426.
MLA Wang, L,et al."Mapping of 18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization (FISH)".ACTA PHYTOTAXONOMICA SINICA 42.5(2004):419-426.
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