| Characterization of 13 microsatellite loci developed from Meconopsis horridula | |
Zhao, Yu1; Zhang, Shi-Bao2 ; Yang, Jing3; Zhang, Lu1
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| 2010 | |
| 发表期刊 | GENETICS AND MOLECULAR BIOLOGY
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| 卷号 | 33期号:3页码:539-541 |
| 摘要 | Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs. |
| 关键词 | Genetic Structure Heterozygosity Meconopsis Horridula Microsatellite Markers Polymorphism |
| 学科领域 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281913300026 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/11223 |
| 专题 | 中国西南野生生物种质资源库 |
| 作者单位 | 1.Jiangxi Agr Univ, Coll Landscape & Art, Nanchang 330045, Jiangxi, Peoples R China 2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,et al. Characterization of 13 microsatellite loci developed from Meconopsis horridula[J]. GENETICS AND MOLECULAR BIOLOGY,2010,33(3):539-541. |
| APA | Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,&Zhang, Lu.(2010).Characterization of 13 microsatellite loci developed from Meconopsis horridula.GENETICS AND MOLECULAR BIOLOGY,33(3),539-541. |
| MLA | Zhao, Yu,et al."Characterization of 13 microsatellite loci developed from Meconopsis horridula".GENETICS AND MOLECULAR BIOLOGY 33.3(2010):539-541. |
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