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Characterization of 13 microsatellite loci developed from Meconopsis horridula
Zhao, Yu1; Zhang, Shi-Bao2; Yang, Jing3; Zhang, Lu1
2010
Source PublicationGENETICS AND MOLECULAR BIOLOGY
Volume33Issue:3Pages:539-541
AbstractMeconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.
KeywordGenetic Structure Heterozygosity Meconopsis Horridula Microsatellite Markers Polymorphism
Subject AreaBiochemistry & Molecular Biology ; Genetics & Heredity
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000281913300026
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/11223
Collection中国西南野生生物种质资源库
Affiliation1.Jiangxi Agr Univ, Coll Landscape & Art, Nanchang 330045, Jiangxi, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,et al. Characterization of 13 microsatellite loci developed from Meconopsis horridula[J]. GENETICS AND MOLECULAR BIOLOGY,2010,33(3):539-541.
APA Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,&Zhang, Lu.(2010).Characterization of 13 microsatellite loci developed from Meconopsis horridula.GENETICS AND MOLECULAR BIOLOGY,33(3),539-541.
MLA Zhao, Yu,et al."Characterization of 13 microsatellite loci developed from Meconopsis horridula".GENETICS AND MOLECULAR BIOLOGY 33.3(2010):539-541.
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