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题名: Characterization of 13 microsatellite loci developed from Meconopsis horridula
作者: Zhao, Yu1; null(张石宝)2; Yang, Jing3; Zhang, Lu1
刊名: GENETICS AND MOLECULAR BIOLOGY
关键词: genetic structure ; heterozygosity ; Meconopsis horridula ; microsatellite markers ; polymorphism
英文摘要: Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.
出版日期: 2010
卷号: 33, 期号:3, 页码:539-541
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Genetics & Heredity
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/11223
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Jiangxi Agr Univ, Coll Landscape & Art, Nanchang 330045, Jiangxi, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China

Recommended Citation:
Zhao, Y; Zhang, SB; Yang, J; Zhang, L.Characterization of 13 microsatellite loci developed from Meconopsis horridula,GENETICS AND MOLECULAR BIOLOGY,2010,33(3):539-541
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