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Characterization of 13 microsatellite loci developed from Meconopsis horridula
Zhao, Yu1; Zhang, Shi-Bao2; Yang, Jing3; Zhang, Lu1
2010
发表期刊GENETICS AND MOLECULAR BIOLOGY
卷号33期号:3页码:539-541
摘要Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.
关键词Genetic Structure Heterozygosity Meconopsis Horridula Microsatellite Markers Polymorphism
资助信息Germplasm Bank of Wild Species, China ; Natural Science Foundation of China [30770226]; Natural Science Foundation of Yunnan [2006C0043Q]; West Light Foundation of the Chinese Academy of Sciences
学科领域Biochemistry & Molecular Biology ; Genetics & Heredity
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号WOS:000281913300026
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/11223
专题中国西南野生生物种质资源库
作者单位1.Jiangxi Agr Univ, Coll Landscape & Art, Nanchang 330045, Jiangxi, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Kunming, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China
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Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,et al. Characterization of 13 microsatellite loci developed from Meconopsis horridula[J]. GENETICS AND MOLECULAR BIOLOGY,2010,33(3):539-541.
APA Zhao, Yu,Zhang, Shi-Bao,Yang, Jing,&Zhang, Lu.(2010).Characterization of 13 microsatellite loci developed from Meconopsis horridula.GENETICS AND MOLECULAR BIOLOGY,33(3),539-541.
MLA Zhao, Yu,et al."Characterization of 13 microsatellite loci developed from Meconopsis horridula".GENETICS AND MOLECULAR BIOLOGY 33.3(2010):539-541.
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