| An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing | |
Shi, Chao1,2; Hu, Na1,2; Huang, Hui1; Gao, Ju1; Zhao, You-Jie1; Gao, Li-Zhi1
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| 通讯作者 | Shi, C (reprint author), Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China, lgao@mail.kib.ac.cn |
| 2012-02-22 | |
| 发表期刊 | PLOS ONE
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| ISSN | 1932-6203 |
| 卷号 | 7期号:2页码:e31468 |
| 摘要 | Background: Chloroplast genomes supply valuable genetic information for evolutionary and functional studies in plants. The past five years have witnessed a dramatic increase in the number of completely sequenced chloroplast genomes with the application of second-generation sequencing technology in plastid genome sequencing projects. However, costeffective high-throughput chloroplast DNA (cpDNA) extraction becomes a major bottleneck restricting the application, as conventional methods are difficult to make a balance between the quality and yield of cpDNAs. |
| 关键词 | Itraq Technology Neural Cell Adhesion Molecule 1 Neurodegenerative Diseases Tianma Gastrodia-elata Blume Prolyl Isomerase Pin1 Neurodegenerative Diseases Alzheimers-disease Neurotrophic Factor Retinoic Acid Triosephosphate Isomerase Cis/trans Isomerases Ether Fraction Sh-sy5y Cells |
| 学科领域 | Biology |
| DOI | 10.1371/journal.pone.0031468 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000302875500021 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/10541 |
| 专题 | 中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Chao,Hu, Na,Huang, Hui,et al. An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing[J]. PLOS ONE,2012,7(2):e31468. |
| APA | Shi, Chao,Hu, Na,Huang, Hui,Gao, Ju,Zhao, You-Jie,&Gao, Li-Zhi.(2012).An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing.PLOS ONE,7(2),e31468. |
| MLA | Shi, Chao,et al."An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing".PLOS ONE 7.2(2012):e31468. |
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