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An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing
Shi, Chao1,2; Hu, Na1,2; Huang, Hui1; Gao, Ju1; Zhao, You-Jie1; Gao, Li-Zhi1
2012-02-22
发表期刊PLOS ONE
ISSN1932-6203
卷号7期号:2页码:e31468
摘要Background: Chloroplast genomes supply valuable genetic information for evolutionary and functional studies in plants. The past five years have witnessed a dramatic increase in the number of completely sequenced chloroplast genomes with the application of second-generation sequencing technology in plastid genome sequencing projects. However, costeffective high-throughput chloroplast DNA (cpDNA) extraction becomes a major bottleneck restricting the application, as conventional methods are difficult to make a balance between the quality and yield of cpDNAs.
关键词Itraq Technology Neural Cell Adhesion Molecule 1 Neurodegenerative Diseases Tianma Gastrodia-elata Blume Prolyl Isomerase Pin1 Neurodegenerative Diseases Alzheimers-disease Neurotrophic Factor Retinoic Acid Triosephosphate Isomerase Cis/trans Isomerases Ether Fraction Sh-sy5y Cells
资助信息National Science Foundation of China[U0936603]; Natural Science Foundation of Yunnan Province[2008CC016]; Chinese Academy of Sciences (CAS)[KSCX2-YW-N-029]; Talent Program of Yunnan Province[20080A009]; Chinese Department of Science and Technology[2007CB815703]; Kunming Institute of Botany, CAS[672705232515]; Kunming Institute of Botany, CAS
学科领域Biology
DOI10.1371/journal.pone.0031468
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000302875500021
引用统计
被引频次:50[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10541
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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GB/T 7714
Shi, Chao,Hu, Na,Huang, Hui,et al. An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing[J]. PLOS ONE,2012,7(2):e31468.
APA Shi, Chao,Hu, Na,Huang, Hui,Gao, Ju,Zhao, You-Jie,&Gao, Li-Zhi.(2012).An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing.PLOS ONE,7(2),e31468.
MLA Shi, Chao,et al."An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing".PLOS ONE 7.2(2012):e31468.
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