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题名: An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing
作者: Shi, Chao1, 2; Hu, Na1, 2; Huang, Hui1; Gao, Ju1; Zhao, You-Jie1; Gao, Li-Zhi1
刊名: PLOS ONE
关键词: iTRAQ technology ; Neural cell adhesion molecule 1 ; Neurodegenerative diseases ; Tianma ; GASTRODIA-ELATA BLUME ; PROLYL ISOMERASE PIN1 ; NEURODEGENERATIVE DISEASES ; ALZHEIMERS-DISEASE ; NEUROTROPHIC FACTOR ; RETINOIC ACID ; TRIOSEPHOSPHATE ISOMERASE ; CIS/TRANS ISOMERASES ; ETHER FRACTION ; SH-SY5Y CELLS
英文摘要: Background: Chloroplast genomes supply valuable genetic information for evolutionary and functional studies in plants. The past five years have witnessed a dramatic increase in the number of completely sequenced chloroplast genomes with the application of second-generation sequencing technology in plastid genome sequencing projects. However, costeffective high-throughput chloroplast DNA (cpDNA) extraction becomes a major bottleneck restricting the application, as conventional methods are difficult to make a balance between the quality and yield of cpDNAs.
出版日期: 2012-02-22
卷号: 7, 期号:2, 页码:e31468
DOI标识: 10.1371/journal.pone.0031468
语种: 英语
收录类别: SCI
学科分类: Biology
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10541
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Shi, C; Hu, N; Huang, H; Gao, J; Zhao, YJ; Gao, LZ.An Improved Chloroplast DNA Extraction Procedure for Whole Plastid Genome Sequencing,PLOS ONE,2012,7(2):e31468
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