| Pseudolaric acid B induces apoptosis via proteasome-mediated Bcl-2 degradation in hormone-refractory prostate cancer DU145 cells | |
Zhao, Dandan1,2; Lin, Feng1; Wu, Xingde3; Zhao, Qinshi3 ; Zhao, Binjiahui1; Lin, Ping1; Zhang, Yanlong2; Yu, Xiaoguang1
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| 通讯作者 | Yu, XG (reprint author), Harbin Med Univ, Coll Basic Med Sci, Dept Biochem & Mol Biol, 194 Xue Fu Rd, Harbin 150081, Peoples R China, zhao_dan_dan@yahoo.com.cn ; xiaolin_linfeng@yahoo.com.cn ; wuxingde@mail.kib.ac.cn ; zhaoqinshi@mail.kib.ac.cn ; jiahuismn@163.com ; lp1995hyd@126.com ; zhangylhd@yahoo.com.cn ; xiaoguang_yu@yahoo.com |
| 2012-06-01 | |
| 发表期刊 | TOXICOLOGY IN VITRO
 |
| ISSN | 0887-2333 |
| 卷号 | 26期号:4页码:595-602 |
| 摘要 | Pseudolaric acid B (PAB), a natural diterpene acid present in the traditional Chinese medicinal herb Tu-Jin-Pi, exerted anticancer effects on various cancer cells. However, the effect of PAB on DU145 cells, an in vitro model of hormone-refractory prostate cancer (HRPC), has not been reported previously. In the study. PAB significantly suppressed proliferation of DU145 cells in a dose-dependent manner without obvious cytotoxicity. IC50 values of 0.89 +/- 0.18 and 0.76 +/- 0.151 mu M at 48 h was determined by Cell counting kit (CCK-8) assay and clone formation assay, respectively. PAB also induced DU145 cells apoptosis as confirmed by typical morphological changes and Annexin V-FITC staining. Furthermore, we demonstrated that PAB caused a concentration-dependent elevation of reactive oxygen species (ROS) level in DU145 cells, and N-acetyl-L-cysteine (NAC, a well-known ROS scavenger) could block PAB-induced ROS generation and apoptosis. Western blotting and/or caspase activity data indicated that PAB downregulated anti-apoptotic Bcl-2 protein and activated caspase-9 and caspase-3, which were largely rescued by NAC or MG-132 (proteasome inhibitor). Taken together, these findings provide the first evidence that PAB may inhibit growth of HRPC DU145 cells and induce apoptosis through ROS generation and Bcl-2 degradation via the activation of the ubiquitin-proteasome pathway. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Pseudolaric Acid b Hormone-refractory Prostate Cancer Apoptosis Bcl-2 Degradation Proteasome Reactive Oxygen Species |
| 学科领域 | Toxicology |
| DOI | 10.1016/j.tiv.2012.02.004 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000303702900007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/10455 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Harbin Med Univ, Coll Basic Med Sci, Dept Biochem & Mol Biol, Harbin 150081, Peoples R China 2.Heilongjiang Univ, Coll Life Sci, Prov Key Lab Mol Biol, Harbin 150086, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Heilongtan 650204, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Dandan,Lin, Feng,Wu, Xingde,et al. Pseudolaric acid B induces apoptosis via proteasome-mediated Bcl-2 degradation in hormone-refractory prostate cancer DU145 cells[J]. TOXICOLOGY IN VITRO,2012,26(4):595-602. |
| APA | Zhao, Dandan.,Lin, Feng.,Wu, Xingde.,Zhao, Qinshi.,Zhao, Binjiahui.,...&Yu, Xiaoguang.(2012).Pseudolaric acid B induces apoptosis via proteasome-mediated Bcl-2 degradation in hormone-refractory prostate cancer DU145 cells.TOXICOLOGY IN VITRO,26(4),595-602. |
| MLA | Zhao, Dandan,et al."Pseudolaric acid B induces apoptosis via proteasome-mediated Bcl-2 degradation in hormone-refractory prostate cancer DU145 cells".TOXICOLOGY IN VITRO 26.4(2012):595-602. |
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